Method validation for determination of trace elements in urine for occupational evaluation of foundry workers

• Grant number: 17/20914-8
• Support Opportunities: Scholarships in Brazil - Master
• Start date: October 01, 2018
• End date: September 30, 2019
• Field of knowledge: Health Sciences - Pharmacy - Toxicological Analysis
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Bruno Lemos Batista
• Grantee: Bruna Moreira Freire
• Host Institution: Centro de Ciências Naturais e Humanas (CCNH). Universidade Federal do ABC (UFABC). Ministério da Educação (Brasil). Santo André , SP, Brazil
• Associated research grant: 14/05151-0 - Arsenic and rice: monitoring and (bio)remediation studies for food safety, AP.JP

Abstract

The trace elements are classified as essential and non-essential. The essential are necessary for the functioning of the organism. Nonessentials, besides being unnecessary, are potentially toxic. Occupational exposure to these elements can cause numerous injuries to workers, and this risk makes it necessary to develop techniques for monitoring their concentrations in biological samples. Inductively coupled plasma mass spectrometry (ICP-MS) is an analytical technique of high sensitivity, precision and reliability that allows rapid and multi-element analysis and is ideal for the analysis of metals in clinical samples. There are several studies of the application of ICP-MS in blood, plasma and urine analysis. Urine, according to NR 7, is the most appropriate biological fluid for the analysis of Arsenic, Cadmium, Lead, Chromium and Mercury. There are studies of the application of ICP-MS for determination of other metals in urine, such as Iron, Copper, Zinc, Selenium, Calcium, Magnesium, Aluminum, Barium, Beryllium, Cobalt, Cesium, Manganese, Nickel, Platinum, Antimony, Tin , Tellurium and Uranium. In these studies, the samples are treated with simple dilution and direct injection in ICP-MS. The objective of this project is the development, validation and application of a method for determination of trace elements in workers' urine via ICP-MS. With this, it is possible to investigate a possible relationship between the concentration of metals in workers' urine and occupational exposure, as well as to determine possible sources of contamination and deficiencies of essential elements. For the development of the method, the following parameters will be evaluated: sample dilution, optimization of ICP-MS variables, evaluation of the use of internal standards and evaluation of calibration in aqueous medium or by matrix adjustment. The method developed will be validated through the study of linearity, limits of detection and quantification, repeatability, reproducibility, precision and accuracy. Workers from the foundry industry of ferrous and non-ferrous metals will participate in the study. They should collect urine in metal-free vials in the morning, and store it under refrigeration. The collected urine will be diluted in acid medium and analyzed by ICP-MS.

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

KOHATSU, MARCIO YUKIHIRO; PELEGRINO, MILENA TREVISAN; MONTEIRO, LUCILENA REBELO; FREIRE, BRUNA MOREIRA; PEREIRA, RODRIGO MENDES; FINCHEIRA, PAOLA; RUBILAR, OLGA; TORTELLA, GONZALO; BATISTA, BRUNO LEMOS; DE JESUS, TATIANE ARAUJO; et al. Comparison of foliar spray and soil irrigation of biogenic CuO nanoparticles (NPs) on elemental uptake and accumulation in lettuce. APL BIOENGINEERING, v. 5, n. 1, p. 16350-16367, MAR 1 2021. (19/26337-8, 16/10060-9, 17/05029-8, 17/20914-8, 18/08194-2)

KOHATSU, MARCIO YUKIHIRO; LANGE, CAMILA NEVES; PELEGRINO, MILENA TREVISAN; PIERETTI, JOANA CLAUDIO; TORTELLA, GONZALO; RUBILAR, OLGA; BATISTA, BRUNO LEMOS; SEABRA, AMEDEA BAROZZI; DE JESUS, TATIANE ARAUJO. Foliar spraying of biogenic CuO nanoparticles protects the defence system and photosynthetic pigments of lettuce (Lactuca sativa). JOURNAL OF CLEANER PRODUCTION, v. 324, NOV 15 2021. (19/26337-8, 17/20914-8, 17/05029-8, 21/00738-6, 18/08194-2, 16/10060-9)

FREIRE, BRUNA MOREIRA; LANGE, CAMILA NEVES; PEDRON, TATIANA; MONTEIRO, LUCILENA REBELO; SANCHES, LIVIA RENTAS; PEDREIRA-FILHO, WALTER DOS REIS; BATISTA, BRUNO LEMOS. Occupational exposure of foundry workers assessed by the urinary concentrations of 18 elements and arsenic species. JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY, v. 62, DEC 2020. (14/05151-0, 16/10060-9, 17/20914-8)

FREIRE, BRUNA MOREIRA; GONZAGA, ROBERTA GRANJA; PEDRON, TATIANA; MONTEIRO, LUCILENA REBELO; LANGE, CAMILA NEVES; PEDREIRA FILHO, WALTER DOS REIS; BATISTA, BRUNO LEMOS. Occupational exposure to potentially toxic elements in the foundry industry: an integrated environmental and biological monitoring. Environmental Science and Pollution Research, v. 28, n. 26, SI, p. 34630-34641, JUL 2021. (16/19924-6, 17/20914-8, 20/00284-2, 16/10060-9, 14/05151-0)

KOHATSU, MARCIO YUKIHIRO; PELEGRINO, MILENA TREVISAN; MONTEIRO, LUCILENA REBELO; FREIRE, BRUNA MOREIRA; PEREIRA, RODRIGO MENDES; FINCHEIRA, PAOLA; RUBILAR, OLGA; TORTELLA, GONZALO; BATISTA, BRUNO LEMOS; DE JESUS, TATIANE ARAUJO; et al. Comparison of foliar spray and soil irrigation of biogenic CuO nanoparticles (NPs) on elemental uptake and accumulation in lettuce. Environmental Science and Pollution Research, v. 28, n. 13, p. 16350-16367, APR 2021. (17/05029-8, 19/26337-8, 16/10060-9, 17/20914-8, 18/08194-2)

LANGE, CAMILA NEVES; MONTEIRO, LUCILENA REBELO; FREIRE, BRUNA MOREIRA; FRANCO, DANIEL FERNANDEZ; DE SOUZA, ROGERIO OLIVEIRA; DOS REIS FERREIRA, CECILIA SACRAMENTO; CENTENO DA SILVA, JULIO JOSE; BATISTA, BRUNO LEMOS. Mineral profile exploratory analysis for rice grains traceability. Food Chemistry, v. 300, DEC 1 2019. (17/20914-8, 18/21494-5, 14/05151-0)

PEDRON, TATIANA; FREIRE, BRUNA MOREIRA; CASTRO, CARLOS EDUARDO; RIBAL, LUIZ FELIPE; BATISTA, BRUNO LEMOS. Availability of arsenic in rice grains by in vitro and in vivo (humans) assays. JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY, v. 56, p. 184-191, DEC 2019. (17/20914-8, 16/10060-9, 14/05151-0, 16/19924-6)

FREIRE, BRUNA MOREIRA; SANTOS, VIVIAN DA SILVA; FERREIRA NEVES, PERICLES DE CARVALHO; OLIVEIRA SOUZA REIS, JULIANA MARIA; DE SOUZA, SAMUEL SIMIAO; BARBOSA JR, FERNANDO; BATISTA, BRUNO LEMOS. Elemental chemical composition and As speciation in rice varieties selected for biofortification. ANALYTICAL METHODS, v. 12, n. 16, p. 2102-2113, APR 28 2020. (17/20914-8, 10/07566-1, 14/05151-0)