In vitro bioaccessibility assessment and in vivo bioavailability of arsenic ingest...
| Full text | |
| Author(s): |
Pedron, Tatiana
[1]
;
Freire, Bruna Moreira
[1]
;
Castro, Carlos Eduardo
[1]
;
Ribal, Luiz Felipe
[2]
;
Batista, Bruno Lemos
[1]
Total Authors: 5
|
| Affiliation: | [1] Fed Univ ABC, Ctr Nat & Human Sci, BR-09210580 Santo Andre, SP - Brazil
[2] Fed Univ ABC, Ctr Engn Modeling & Appl Social Sci, BR-09210580 Santo Andre, SP - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY; v. 56, p. 184-191, DEC 2019. |
| Web of Science Citations: | 0 |
| Abstract | |
Background: Rice grains are consumed by approximately half of the world's population. This cereal has higher arsenic (As) concentrations in grains than wheat or barley. Arsenic determination in food and/or in vitro studies are important for risk assessment; however, it is not enough to assess the real human exposure. Method: In vitro bioaccessibility was carried out in husked-rice using gastric and intestinal solutions similar to humans. Also, As naturally found in husked-rice was evaluated by in vivo bioavailability in humans. For this purpose, diets from the 1st and 2nd days were free of foods known to be high in As; 3rd and 4th days the diets were composed by rice and water and; 5th and 6th the diet was similar the 1st and 2nd days. During all experimentation, a representative aliquot of each meal, blood and urine were collected for total As (t-As) determination. Arsenic species were determined in the urine. Results: t-As in husked rice varied from 157.3 +/- 30.6 to 240.2 +/- 85.2 mu g kg(-1). The in vitrobioaccessible fractions ranged from 91 to 94%. Inorganic As (i-As) ranged from 99.7 +/- 11.2 to 159.5 +/- 29.4 mu g kg(-1). For the in vivo assay, t-As concentrations in the woman and man blood were about 3 mu g mL(-1) from the 1st to 6th day. Arsenic from the rice ingested was excreted by urine about 72 h after ingestion. The t-As and dimethyl As (DMA) in urine ranged from 3.59 to 47.17 and 1.02 to 2.55 mu g g(-1) creatinine for the volunteers, indicating a two-fold DMA-increase in urine after ingestion of husked-rice. Conclusion: After rice ingestion, As was quickly metabolized. The higher As concentrations were found in urine 72 h after rice ingestion. The main As-specie found in urine was DMA, indicating that methylation of As from rice followed by urine excretion is the main biological pathway for As excretion. (AU) | |
| FAPESP's process: | 17/20914-8 - Method validation for determination of trace elements in urine for occupational evaluation of foundry workers |
| Grantee: | Bruna Moreira Freire |
| Support type: | Scholarships in Brazil - Master |
| FAPESP's process: | 16/10060-9 - Multi-user equipment (MUE) approved in Grant FAPESP project 2014/05151-0: inductively coupled plasma mass spectrometer hyphenated to an high performance liquid chromatography (HPLC-ICP-MS) |
| Grantee: | Bruno Lemos Batista |
| Support type: | Multi-user Equipment Program |
| FAPESP's process: | 14/05151-0 - Arsenic and rice: monitoring and (bio)remediation studies for food safety |
| Grantee: | Bruno Lemos Batista |
| Support type: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 16/19924-6 - In vitro bioaccessibility assessment and in vivo bioavailability of arsenic ingested through rice commonly consumed by Brazilians |
| Grantee: | Tatiana Pedron |
| Support type: | Scholarships in Brazil - Doctorate |