Study of aggressiveness prediction of mammary adenocarcinoma by Raman spectroscopy

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Author(s):	Bitar, Renata Andrade ; Martinho, Herculano da Silva ; Zambelli Ramalho, Leandra Naira ; dos Santos Junior, Arnaldo Rodrigues ; Ramalho, Fernando Silva ; Raniero, Leandro ; Martin, Airton A. ; MahadevanJansen, A ; Petrich, W Total Authors: 9
Document type:	Journal article
Source:	BIOMEDICAL VIBRATIONAL SPECTROSCOPY V: ADVANCES IN RESEARCH AND INDUSTRY; v. 8219, p. 7-pg., 2012-01-01.
Abstract
Although there are many articles focused on in vivo or ex vivo Raman analysis for cancer diagnosis, to the best of our knowledge its potential to predict the aggressiveness of tumor has not been fully explored yet. In this work Raman spectra in the finger print region of ex vivo breast tissues of both healthy mice (normal) and mice with induced mammary gland tumors (abnormal) were measured and associated to matrix metalloproteinase-19 (MMP-19) immunohistochemical exam. It was possible to verify that normal breast, benign lesions, and adenocarcinomas spectra, including the subtypes (cribriform, papillary and solid) could have their aggressiveness diagnosed by vibrational Raman bands. By using MMP-19 exam it was possible to classify the samples by malignant graduation in accordance to the classification results of Principal Component Analysis (PCA). The spectra NM/MH were classified correctly in 100% of cases; CA/CPA group had 60% of spectra correctly classified and for PA/AS 54% of the spectra were correctly classified. (AU)