Diagnosing basal cell carcinoma in vivo by near-infrared Raman spectroscopy: a Principal Components Analysis discrimination algorithm

Author(s):Show less -

Silveira, Landulfo, Jr. ; Silveira, Fabricio L. ; Bodanese, Benito ; Pacheco, Marcos Tadeu T. ; Zangaro, Renato A. ; Kollias, N ; Choi, B ; Zeng, H ; Kang, HW ; Knudsen, BE ; Wong, BJF ; Ilgner, JF ; Izdebski, K ; Suter, MJ ; Lam, S ; Brenner, M ; Gregory, KW ; Tearney, GJ ; Marcu, L ; Hirschberg, H ; Madsen, S ; MahadevanJansen, A ; Jansen, ED ; Mandelis, A

• Document type: Journal article
• Source: PHOTONIC THERAPEUTICS AND DIAGNOSTICS VIII, PTS 1 AND 2; v. 8207, p. 7-pg., 2012-01-01.

Abstract

This work demonstrated the discrimination among basal cell carcinoma (BCC) and normal human skin in vivo using near-infrared Raman spectroscopy. Spectra were obtained in the suspected lesion prior resectional surgery. After tissue withdrawn, biopsy fragments were submitted to histopathology. Spectra were also obtained in the adjacent, clinically normal skin. Raman spectra were measured using a Raman spectrometer (830 nm) with a fiber Raman probe. By comparing the mean spectra of BCC with the normal skin, it has been found important differences in the 800-1000 cm(-1) and 1250-1350 cm(-1) (vibrations of C-C and amide III, respectively, from lipids and proteins). A discrimination algorithm based on Principal Components Analysis and Mahalanobis distance (PCA/MD) could discriminate the spectra of both tissues with high sensitivity and specificity. (AU)

• FAPESP's process: 10/11111-0 - Diagnostic model of carcinoma and melanoma of human skin in vivo by dispersive RAMAM spectroscopy
• Grantee: Fabricio Luiz Silveira
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 09/01788-5 - Dispersive Raman spectroscopy using optical fibers "Raman probe" applied to the diagnosis of neoplasia in the skin and prostate
• Grantee: Landulfo Silveira Junior
• Support Opportunities: Regular Research Grants