Dendritic cells and T lymphocytes interactions in a novel 3D system

Author(s)

Steponavicius-Cruz, Karen ; Freitas, Vanessa Morais ; Barbuto, Jose Alexandre M. ; Bartolo, P ; Fernandes, P

• Document type: Journal article
• Source: X INTERNATIONAL CONFERENCE ON STRUCTURAL DYNAMICS (EURODYN 2017); v. 59, p. 8-pg., 2013-01-01.

Abstract

We describe the interactions between monocyte-derived DCs, in different stages of maturation, with allogeneic T lymphocytes in a 3D system. Maturation of DCs increased their interaction time with T lymphocytes from 43 to 138 minutes. The average motility of T lymphocytes interacting or not with DCs was also affected, varying from 0.21 mu m-0.37 mu m/minute to 0.36 mu m-0.52 mu m/minute. These data indicate that this 3D Biotek (TM) scaffold enables interactions between lymphocytes and DCs at different stages of maturation and may be useful for the characterization of these interactions, the cellular subtypes and patterns of response induced. (C) 2013 The Authors. Published by Elsevier Ltd. (AU)

• FAPESP's process: 10/18139-7 - Cellular interactions in three-dimensional environment between dendritic cell-tumor cell hybrids and human lymphocytes: in search of strategies for enhancing antitumor vaccines
• Grantee: Karen Steponavicius Piedade Cruz
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 09/54599-5 - Dendritic cells: integrative elements of the immune system - an applied approach
• Grantee: Jose Alexandre Marzagão Barbuto
• Support Opportunities: Research Projects - Thematic Grants