Experimental host range of Citrus leprosis virus C (CiLV-C)

Author(s)

Garita, Laura Cristina ; Tassi, Aline Daniele ; Calegario, Renata Faier ; Freitas-Astua, Juliana ; Salaroli, Renato B. ; Romao, Gerson O. ; Kitajima, Elliot W.

• Document type: Journal article
• Source: ROPICAL PLANT PATHOLOG; v. 39, n. 1, p. 13-pg., 2014-01-01.

Abstract

Citrus leprosis (CL) is a serious threat to the citrus industry, especially for sweet oranges. For a long time, Citrus spp. were considered the only susceptible hosts. However, other plant species were also found either experimentally or naturally to be susceptible to Citrus leprosis virus C (CiLV-C). To assess the experimental host range of CiLV-C, a large number of plant species were inoculated with Brevipalpus phoenicis, viruliferous to CiLV-C, under experimental conditions. Out of the 140 tested species (43 families), 59 species (24 families) developed localized chlorotic and/or necrotic lesions upon inoculation of leaves with viruliferous mites, and 40 species (18 families) of them yielded positive results for CiLV-C detection in at least one of the following assays: ELISA, RT-PCR, transmission electron microscopy and immunfluorescence. For those that developed lesions and yielded negative results in CiLV-C detection assays, the results may be attributed to the small number of lesions and their necrotic state with very little viral material. The fact that a considerable number of plant species are susceptible to the virus after mite inoculation brings up implications for the epidemiology, quarantine and evolution of the citrus leprosis pathosystem. (AU)

• FAPESP's process: 08/52691-9 - Management of citrus leprosis
• Grantee: Elliot Watanabe Kitajima
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 11/14496-2 - Evaluation of the morphological and genomic diversity of different populations of Brevipalpus (Acari: Tenuipalpidae) species transmitting plant viruses and their ability as vector
• Grantee: Aline Daniele Tassi
• Support Opportunities: Scholarships in Brazil - Master