Profile of IL-2, IL-4, IL-10, IFN-gamma, TNF-alpha and KC-like cytokines in pregnant bitches

Author(s)

Feliciano, M. A. R. ; Silva, A. S. L. ; Crivelaro, R. M. ; Oliveira, M. E. F. ; Coutinho, L. N. ; Vicente, W. R. R.

• Document type: Journal article
• Source: Arquivo Brasileiro de Medicina Veterinária e Zootecnia; v. 66, n. 4, p. 6-pg., 2014-08-01.

Abstract

The aim of this study was to determine the profile of IL-2, IL-4, IL-10, IFN-gamma, and TNF-alpha cytokines and KC-like cells (natural killer) in pregnant bitches, unpublished values for the species. A total of 27 females of the Shi Tzu, Pug, English Bulldog and French breeds, weighing 4-20kg and aged 4-6 years were used. Blood samples were collected from bitches during the anestrous and on the 2nd, 5th, 6th, 7th and 8th week of pregnancy. Serum levels of cytokines were measured by panel MILLIPLEX MAP (CCYTO-90K, MILLIPORE, Billerica, Massachusetts, USA) validated for dogs. Twenty four females showed physiological pregnancy and three bitches showed pathological pregnancy. There was no difference between cytokine values during anestrous and gestational weeks of bitches (P>0.05). However, it was possible to verify the physiological behavior of serum levels during modulation of immune response in the gestational process of animals. In animals with gestational disorders, abnormal values for IL-2, IL-4 and INF-y were noted. It was concluded that serum levels of cytokines evaluated in pregnant bitches can help the better understanding of physiological and pathological gestational processes and correlated immunology in this species. (AU)

• FAPESP's process: 11/06011-9 - Conventional ultrasound and Doppler in maternal-fetal prenatal evaluation of pregnant bitches
• Grantee: Wilter Ricardo Russiano Vicente
• Support Opportunities: Regular Research Grants
• FAPESP's process: 10/16913-7 - Conventional ultrassonography and Dopplerflow maternal fetal prenatal in evaluation of pregnant bitches
• Grantee: Marcus Antônio Rossi Feliciano
• Support Opportunities: Scholarships in Brazil - Post-Doctoral