Characterization of M1 and M2 monocyte subpopulations and their association with cytokine production in pregnant women with preeclampsia

• Grant number: 09/11924-3
• Support Opportunities: Scholarships in Brazil - Master
• Effective date (Start): March 01, 2010
• Effective date (End): February 29, 2012
• Field of knowledge: Biological Sciences - Immunology - Applied Immunology
• Principal Investigator: Maria Terezinha Serrão Peraçoli
• Grantee: Leonardo Teixeira Lopes de Medeiros
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Monocytes from pregnant women with preeclampsia are endogenously activated and secrete high levels of free radicals and inflammatory cytokines. The objectives of this study are: 1) To examine whether the inflammatory state of monocytes observed in preeclamptic patients is associated with polarization of M1 subset profile in peripheral blood monocytes, correlating the expression of surface markers CD64, TLR2, TLR4 (M1 subset) and CD163 and CD206 (M2 subset) with cytokine production; 2) to differentiate between early-onset preeclampsia and late-onset preeclampsia by M1 and M2 monocyte characterization. Ninety pregnant women, 30 normotensive and 60 preeclamptic pregnant women will be paired by gestational age. Pregnant women with preeclampsia will be classified according to the onset of clinical manifestations, before 34 weeks of gestation (early-onset preeclampsia) and from 34 weeks of gestation on(late-onset preeclampsia). Peripheral blood monocytes obtained from normotensive and preeclamptic women will be evaluated in relation to surface markers of M1 inflammatory monocytes (TLR2, TLR4 and CD64-FcgR1) and M2 suppressor monocytes (CD206-mannose receptor and CD163-scavenger receptor) by flow cytometry. Pro-inflammatory cytokines associated with M1 profile (TNF-alpha, IL-6, IL-12, IL-23) and anti-inflammatory cytokines (IL-10 and TGF-beta1) associated with M2 profile will be determined in monocyte cultured with or without lypopolisaccharide of Escherichia coli (LPS) or peptidoglycan (PG) of Gram-negative bacterium by enzyme immunoassay after 4h and 18 h of culture. The expression of monocyte surface markers will be determined in three periods: immediately after blood collection (endogenous expression), after 4h and 18h of culture with or without LPS or PG (stimulated expression). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MEDEIROS, LEONARDO T. L.; PERACOLI, JOSE C.; BANNWART-CASTRO, CAMILA F.; ROMAO, MARIANA; WEEL, INGRID C.; GOLIM, MARJORIE A.; DE OLIVEIRA, LEANDRO G.; KUROKAWA, CILMERY S.; MEDEIROS BORGES, VERA T.; PERACOLI, MARIA T. S.. Monocytes from Pregnant Women with Pre-Eclampsia are Polarized to a M1 Phenotype. American Journal of Reproductive Immunology, v. 72, n. 1, p. 5-13, JUL 2014. (09/11924-3)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MEDEIROS, Leonardo Teixeira Lopes de. Caracterização das subpopulações de monócitos M1 e M2 e associação com produção de citocinas em gestantes portadoras de pré-eclâmpsia. 2011. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Medicina. Botucatu Botucatu.