Cecropia pachystachya extract attenuated the renal lesion in 5/6 nephrectomized rats by reducing inflammation and renal arginase activity

Author(s)

Maquiaveli, Claudia C. ; da Silva, Edson R. ; Rosa, Lara C. ; Francescato, Heloisa D. C. ; Lucon Junior, Joao Francisco ; Silva, Cleonice G. A. ; Casarini, Dulce E. ; Ronchi, Fernanda A. ; Coimbra, Terezila M.

• Document type: Journal article
• Source: Journal of Ethnopharmacology; v. 158, p. 9-pg., 2014-12-02.

Abstract

Ethnopharmacological relevance: The plant Cecropia pachystachya Trecul has been used in Brazilian folk medicine to treat hypertension, bladder and kidney inflammation and renal diseases. The aim of this study was to evaluate the potential of the aqueous fraction from the ethanolic extract of Cecropia pachystachya (FCP) in the management of hypertension, inflammation and progressive renal disease in rats submitted to 5/6 nephrectomy. Materials and methods: Thirty male Wistar rats submitted to 5/6 nephrectomy (5/6 NE) were untreated (NE) or treated (NE+FCP) with the FCP (0.5 g/kg/day). The treatment started 15 days after surgery, and the rats were followed for a period of 60 days. Systolic blood pressure (SBP) and albuminuria were evaluated from 15-60 days after the surgical procedure. Function and estructural renal changes, TGF-beta (transforming growth factor beta), MCP-1 (monocyte chemoattractant protein-1) and nitric oxide (NO) urinary excretion were analyzed. Expression and activity of the renal enzymes arginase (ARG), angiotensin converting enzyme (ACE), and MAP kinase p-JNK expression also were analyzed. Results: The nephrectomized rats developed progressive albuminuria and increased SBP that was less intense in the treated group. There was a reduction in the glomerular filtration rate (GFR) in the nephrectomized rats, which was attenuated by treatment with FCP extract. The treatment with FCP also attenuated the histological changes, reduced the expression and activity of renal arginase, the number of macrophages (ED-1 positive cells) and the p-JNK expression in the renal cortex of the rats submitted to 5/6 NE. The urinary excretion of TGF-beta was less intense in the treated group and was associated with the reduction of the expression and activity of the renal arginase. Conclusions: These results suggest that the reduction of renal arginase activity, p-JNK and TGF-beta expression can explain the mechanism by which the treatment with C. pachystachya reduced the inflammation and improved renal function. This study presents the potential use of Cecropia pachystachya in the treatment of chronic renal diseases. (C) 2014 Elsevier Ireland Ltd. All rights reserved. (AU)

• FAPESP's process: 12/17059-5 - Characterization of natural inhibitors and the study of structure-activity relationship of flavonoids based on the arginase enzyme from Leishmania amazonensis
• Grantee: Edson Roberto da Silva
• Support Opportunities: Regular Research Grants