TBX21-1993T/C (rs4794067) polymorphism is associated with increased risk of chronic periodontitis and increased T-bet expression in periodontal lesions, but does not significantly impact the IFN-g transcriptional level or the pattern of periodontophatic bacterial infection

Author(s)

Cavalla, Franco ; Biguettii, Claudia Cristina ; Colavite, Priscila Maria ; Silveira, Elcia Varise ; Martins, Walter, Jr. ; Letra, Ariadne ; Favaro Trombone, Ana Paula ; Silva, Renato Menezes ; Garlet, Gustavo Pompermaier

• Document type: Journal article
• Source: VIRULENCE; v. 6, n. 3, p. 12-pg., 2015-04-01.

Abstract

Th1-polarized host response, mediated by IFN-gamma, has been associated with increased severity of periodontal disease as well as control of periodontal infection. The functional polymorphism TBX21-1993T/C (rs4794067) increases the transcriptional activity of the TBX21 gene (essential for Th1 polarization) resulting in a predisposition to a Th-1 biased immune response. Thus, we conducted a case-control study, including a population of healthy controls (H, n = 218), chronic periodontitis (CP, n = 197), and chronic gingivitis patients (CG, n = 193), to investigate if genetic variations in TBX21 could impact the development of Th1 responses, and consequently influence the pattern of bacterial infection and periodontitis outcome. We observed that the polymorphic allele T was significantly enriched in the CP patients compared to CG subjects, while the H controls demonstrated and intermediate genotype. Also, investigating the putative functionality TBX21-1993T/C in the modulation of local response, we observed that the transcripts levels of T-bet, but not of IFN-gamma, were upregulated in homozygote and heterozygote polymorphic subjects. In addition, TBX21-1993T/C did not influence the pattern of bacterial infection or the clinical parameters of disease severity, being the presence/absence of red complex bacteria the main factor associated with the disease status and the subrogate variable probing depth (PD) in the logistic regression analysis. (AU)

• FAPESP's process: 14/03276-0 - Influence of genetic polymorphisms in the colonization/recolonization patterns in chronic periodontitis patients
• Grantee: Ian Franco Cavalla Ruiz
• Support Opportunities: Scholarships in Brazil - Doctorate