Resistance Training Regulates Cardiac Function through Modulation of miRNA-214

Author(s)

Soares Melo, Stephano Freitas ; Barauna, Valerio Garrone ; Carneiro Junior, Miguel Araujo ; Marchesi Bozi, Luiz Henrique ; Drummond, Lucas Rios ; Natali, Antonio Jose ; de Oliveira, Edilamar Menezes

• Document type: Journal article
• Source: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 16, n. 4, p. 13-pg., 2015-04-01.

Abstract

Aims: To determine the effects of resistance training (RT) on the expression of microRNA (miRNA)-214 and its target in sarcoplasmic reticulum Ca2+-ATPase (SERCA2a), and on the morphological and mechanical properties of isolated left ventricular myocytes. Main methods: Male Wistar rats were divided into two groups (n = 7/group): Control (CO) or trained (TR). The exercise-training protocol consisted of: 4 x 12 bouts, 5x/week during 8 weeks, with 80% of one repetition maximum. Key findings: RT increased the left ventricular myocyte width by 15% and volume by 12%, compared with control animals (p < 0.05). The time to half relaxation and time to peak were 8.4% and 4.4% lower, respectively, in cells from TR group as compared to CO group (p < 0.05). RT decreased miRNA-214 level by 18.5% while its target SERCA2a expression were 18.5% higher (p < 0.05). Significance: Our findings showed that RT increases single left ventricular myocyte dimensions and also leads to faster cell contraction and relaxation. These mechanical adaptations may be related to the augmented expression of SERCA2a which, in turn, may be associated with the epigenetic modification of decreased miRNA-214 expression. (AU)

• FAPESP's process: 10/09438-0 - THERAPEUTIC USE OF microRNA RESPONSIBLE FOR REGULATING CARDIAC CONTRACTILITY ASSOCIATED WITH EXERCISE TRAINING AEROBIC IN RATS WITH HEART FAILURE
• Grantee: Stéphano Freitas Soares Melo
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 09/18370-3 - microRNA expression on normotensive rats heart submitted to different aerobic training and the potential to hypertension therapy.
• Grantee: Edilamar Menezes de Oliveira
• Support Opportunities: Regular Research Grants
• FAPESP's process: 10/50048-1 - Cellular and functional bases of exercise in cardiovascular diseases
• Grantee: Carlos Eduardo Negrão
• Support Opportunities: Research Projects - Thematic Grants