| Full text | |
| Author(s): Show less - |
Magalhaes, Lizandra G.
;
Lima, Thais C.
;
de Paula, Renato G.
;
Morais, Enyara R.
;
Aguiar, Daniela P.
;
Gardinassi, Luiz G.
;
Garcia, Gustavo R.
;
Laurentiz, Rosangela S.
;
Rodrigues, Vanderlei
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Bastos, Jairo K.
;
Filho, Ademar A. S.
;
Yatsuda, Ana P.
;
Cunha, Wilson R.
;
Silva, Marcio L. A.
Total Authors: 14
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| Document type: | Journal article |
| Source: | REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY; v. 26, n. 3, p. 8-pg., 2016-05-01. |
| Abstract | |
Schistosomiasis, a chronic disease that affects million people worldwide, is caused by trematode flukes of the genus Schistosoma. The lack of an anti-schistosomiasis vaccine and massive monotherapy with praziquantel reinforces the need for search and development of new therapeutic drugs. Recently, we demonstrated that the essential oil of Piper cubeba L., Piperaceae, and their derivative dibenzylbutyrolactolic (-)-6,6'-dinitrohinokinin, presents in vitro and in vivo activities against Schistosoma mansoni. Here, we identified changes in the protein expression after exposure to dibenzylbutyrolactolic (-)-6,6'-dinitrohinokinin. We applied two-dimensional gel electrophoresis (2-DE) to S. mansoni soluble protein extracts and observed at least 38 spots to be affected by dibenzylbutyrolactolic (-)-6,6'-dinitrohinokinin. We further identified 25 differentially expressed proteins by mass spectrometry. Enrichment for biological processes and predictive analyses of protein-protein interactions suggest that dibenzylbutyrolactolic (-)-6,6'-dinitrohinokinin targets proteins involved mainly in metabolic processes, especially carbohydrate metabolism. In summary, this study provides an interesting approach to understand the anti-parasitic activity of semi-synthetic (-)-6,6'-dinitrohinokinin a derivative compound from lignan and for the development of new therapy strategies. (C) 2016 Sociedade Brasileira de Farmacognosia. Published by Elsevier Editora Ltda. All rights reserved. (AU) | |
| FAPESP's process: | 09/15207-4 - Evaluation of the effect of lignans and neolignans on Schistosoma mansoni: a proteomic study |
| Grantee: | Lizandra Guidi Magalhães |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 98/14956-7 - Investigacao das atividades analgesica, antiinflamatoria e tripanocida de alguns derivados de cubebina obtidos por sintese parcial. |
| Grantee: | Márcio Luís Andrade e Silva |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 13/00382-0 - PROSPECTING TICK SIALOMES FOR DISCOVERY OF NEW ANTIMICROBIAL COMPONENTS |
| Grantee: | Gustavo Rocha Garcia |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 10/17378-8 - Study of the immunomodulatory activity of lignans and neolignans obtained by partial synthesis and oxidative coupling as a potential in developing new antiparasitic drugs |
| Grantee: | Márcio Luís Andrade e Silva |
| Support Opportunities: | Regular Research Grants |