Immunogenic potential of Rhipicephalus (Boophilus) microplus aquaporin 1 against Rhipicephalus sanguineus in domestic dogs

Author(s)

Evora, Patricia Martinez ; Sanches, Gustavo Seron ; Guerrero, Felix David ; de Leon, Adalberto Perez ; Bechara, Gervasio Henrique

• Document type: Journal article
• Source: REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA; v. 26, n. 1, p. 7-pg., 2017-01-01.

Abstract

This study evaluated a recombinant aquaporin 1 protein of Rhipicephalus (Boophilus) microplus (RmAQP1) as antigen in a vaccine against R. sanguineus. Five dogs were immunized with RmAQP1 (10 mu g) + adjuvant (Montanide) (G1), and five were inoculated with adjuvant only (G2), three times. Twenty-one days after the last immunization, animals of both groups were challenged with R. sanguineus larvae, nymphs and adults, and their biotic potential was compared. Blood samples were collected before each immunization and every 28 days after the last immunization for 10 weeks. Serum antibody titers (IgG) were assessed by ELISA. We observed that: engorgement period of adult females from G1 was 12% shorter than G2; larvae from G1 had 8.7% longer engorgement period than G2 and weighed 7.2% less; nymphs from G1 had 4.5% shorter engorgement period than G2 and weighed 3.6% less; although the antibody titers increased following the second immunization, they rapidly decreased after the third immunization. Results indicated low immunoprotection of RmAQP1 against adult R. sanguineus ticks, and possible efficacy on larvae and nymphs fed on immunized dogs. Further studies should be performed for a full evaluation of the immunoprotection of RmAQP1 against R. sanguineus infestations in dogs. (AU)

• FAPESP's process: 13/09792-7 - EVALUATION OF THE IMMUNOGENIC POTENTIAL OF RHIPICEPHALUS (BOOPHILUS) MICROPLUS AQUAPORIN AGAINST THE RHIPICEPHALUS SANGUINEUS TICK, LATREILLE, 1806 IN DOMESTIC DOGS
• Grantee: Patricia Martinez Évora
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 13/10394-6 - Immunogenic Potential of the Ribosomal Protein P0 Against the Tick Rhipicephalus sanguineus (Acari:Ixodidae)in Dogs
• Grantee: Gustavo Seron Sanches
• Support Opportunities: Scholarships in Brazil - Post-Doctoral