Salivary stress biomarkers and anxiety symptoms in children with and without temporomandibular disorders

Author(s)

Kobayashi, Fernanda Yukie ; Duarte Gaviao, Maria Beatriz ; Salome Marquezin, Maria Carolina ; Affonso Fonseca, Fernando Luiz ; Marangoni Montes, Ana Bheatriz ; Barbosa, Tais de Souza ; Castelo, Paula Midori

• Document type: Journal article
• Source: BRAZILIAN ORAL RESEARCH; v. 31, p. 9-pg., 2017-01-01.

Abstract

The etiology of temporomandibular disorders (TMD), which are considered as a heterogeneous group of psychophysiological disturbances, remains a controversial issue in clinical dentistry. This study aimed to evaluate whether the salivary alpha-amylase (sAA), cortisol levels, and anxiety symptoms differ between children with and without TMD. Initially, 316 young subjects were screened in public schools (nonreferred sample); 76 subjects aged 7-14 years were selected and comprised the TMD and control groups with 38 subjects each matched by sex, age, and the presence/absence of sleep bruxism. Four saliva samples were collected: upon waking, 30 min and 1 h after awakening (fasting), and at night (at 8 PM) on 2 alternate days to examine the diurnal profiles of cortisol and sAA. Anxiety symptoms were screened using the Multidimensional Anxiety Scale for Children (MASC-Brazilian version). Shapiro-Wilk test, Student's t-test/Mann-Whitney U test, and correlation tests were used for data analysis. No significant differences were observed in the salivary cortisol area under the curve (AUC(G) mean +/- SD = 90.22 +/- 63.36 x 94.21 +/- 63.13 mu g/dL/min) and sAA AUC(G) (mean +/- SD = 2544.52 +/- 2142.00 x 2054.03 +/- 1046.89 U/mL/min) between the TMD and control groups, respectively (p > 0.05); however, the clinical groups differed in social anxiety domain (t = 3.759; CI = 2.609, 8.496), separation/panic (t = 2.243; CI = 0.309, 5.217), physical symptoms (U = 433.500), a nd MASC total s core (t = -3.527; CI = -23.062, -6.412), with a power of the test >80% and large effect size (d = 0.80), with no significant correlation between the MASC total score, cortisol, and sAA levels. Although children with TMD scored higher in anxiety symptoms, no difference was observed in the salivary stress biomarkers between children with and without TMD. (AU)

• FAPESP's process: 11/50419-2 - Oral health and behavior: interdisciplinary perspectives and emotional aspects in the health-disease process
• Grantee: Antonio Bento Alves de Moraes
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 10/01447-0 - Orofacial and temporomandibular dysfunction evaluation, bite force and salivary cortisol and amylase levels in children and adolescents
• Grantee: Fernanda Yukie Kobayashi
• Support Opportunities: Scholarships in Brazil - Master