Quality of life evaluation, salivary cortisol and amylase levels and electromyographic activity of masticatory muscles: associations with parafunctional and sucking nutritive habits in children

• Grant number: 07/05760-2
• Support Opportunities: Regular Research Grants
• Field of knowledge: Biological Sciences - Physiology
• Principal Investigator: Maria Beatriz Duarte Gavião
• Grantee: Maria Beatriz Duarte Gavião
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil

Abstract

Assessment of stress salivary markers has gotten increasing interest in biomedical and psychological researches. The free cortisol measurement and, recently, the alpha-amylase activity, have demonstrated to be important biomarkers of the endocrine activity in physical and psicossocial stress conditions. The literature suggests that stress and emotional disturbances may be involved in the etiology of stomatognatic parafunctions, like bruxism and persistent sucking habits in children. In this context, it is important to consider the children"s oral health, because of the large number of orofacial disorders that can compromise the function of stomatognatic system, as well as their comfort and quality of life. Thus, this study project is related with three researches, as well as future subsequent researches that will be done in this area. The first study (Postdoctoral project; Fapesp n. 06/06338-0) will evaluate 150 children in the mixed dentition, aged 6-8 years, divided in two groups according to gender. The following variables will be verified: presence/absence of parafuncional habits (bruxism, nail biting, finger/thumb/lip sucking, pacifier), breast- and bottle-feeding, malocclusion; quality of life evaluation scale; body variables (weight, height and heart rate); electromyography of masticatory muscles and bite force; salivary levels of free cortisol and alpha-amylase; aiming to determined the relation among parafunctional habits and local (occlusion) and central factors (hormones and psychological factors). Second study: the purpose will be to evaluate 30 children of both genders aged 84-95 months (7 years), in the mixed dentition, without history of invasive dental treatment (restoration or tooth extraction) or pain of dental origin. The following variables will be verified: salivary levels of free cortisol and alpha-amylase 30 minutes after awakening (baseline), and before and after dental prophylaxis, as well as heart rate, body variables (weight and height) and hour"s sleep. The third study will evaluate 200 children, aged 8-12 years, from public schools and those who were to start dental treatment at Piracicaba Dental School (UNICAMP). The following variables will be verified: 1- dental variables (fluorosis, gingival health, DMF-T and dmf-t index, dentofacial anomalies; 2- functional variables: signs and symptoms of temporomandibular dysfunction; psychological factors: oral health related to quality of life, evaluated by questionnaires CPQ8-10 and CPQ11-14; 4- salivary levels of cortisol and alpha-amylase. Longitudinally, 40 children who have presented similar dental conditions, but different impressions of their oral health will be re-examined and interviewed intra-individually. In this way, the results will be submitted to descriptive statistical analysis, Shapiro-Wilks-W test, Pearson"s and Spearman"s correlation tests, t-test, linear multiple regression and stepwise logistic regression, where appropriate. The significance level will set at p0.05. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CASTELO, PAULA M.; BARBOSA, TAIS S.; GAVIAO, MARIA BEATRIZ D.. Quality of life evaluation of children with sleep bruxism. BMC ORAL HEALTH, v. 10, 2010. (07/05760-2, 06/06338-0)

CASTELO, PAULA MIDORI; BARBOSA, TAIS DE SOUZA; PEREIRA, LUCIANO JOSE; AFFONSO FONSECA, FERNANDO LUIZ; DUARTE GAVIAO, MARIA BEATRIZ. Awakening salivary cortisol levels of children with sleep bruxism. CLINICAL BIOCHEMISTRY, v. 45, n. 9, p. 651-654, JUN 2012. (07/05760-2, 06/06338-0)

LUCAS, B. DE L.; BARBOSA, T. DE S.; PEREIRA, L. J.; GAVIAO, M. B. D.; CASTELO, P. M.. Electromyographic evaluation of masticatory muscles at rest and maximal intercuspal positions of the mandible in children with sleep bruxism. EUROPEAN ARCHIVES OF PAEDIATRIC DENTISTRY, v. 15, n. 4, p. 6-pg., 2014-08-01. (06/06338-0, 07/05760-2)

FURLAN, N. F.; GAVIAO, M. B. D.; BARBOSA, T. S.; NICOLAU, J.; CASTELO, P. M.. Salivary Cortisol, Alpha-Amylase and Heart Rate Variation in Response to Dental Treatment in Children. Journal of Clinical Pediatric Dentistry, v. 37, n. 1, p. 83-87, FAL 2012. (07/05760-2, 08/05103-4, 06/06338-0)

KOBAYASHI, F. Y.; FURLAN, N. F.; BARBOSA, T. S.; CASTELO, P. M.; GAVIAO, M. B. D.. Evaluation of masticatory performance and bite force in children with sleep bruxism. Journal of Oral Rehabilitation, v. 39, n. 10, p. 776-784, OCT 2012. (07/05760-2, 06/06338-0)