Effect of Previous High Glutamine Infusion on Inflammatory Mediators and Mortality in an Acute Pancreatitis Model

Author(s)

Garib, Ricardo ; Garla, Priscila ; Torrinhas, Raquel S. ; Moretti, Ana I. S. ; Machado, Marcel C. C. ; Waitzberg, Dan L.

• Document type: Journal article
• Source: Mediators of Inflammation; v. 2016, p. 10-pg., 2016-01-01.

Abstract

Parenteral glutamine supplementation in acute inflammatory conditions is controversial. We evaluated the inflammatory and survival responses after parenteral glutamine infusion in sodium taurocholate-induced acute pancreatitis (AP) model. Lewis rats received 1 g/kg parenteral glutamine (n = 42), saline (n = 44), or no treatment (n = 45) for 48 h before AP induction. Blood, lung, and liver samples were collected 2, 12, and 24h after AP to measure serum cytokines levels and tissue heat shock protein (HSP) expression. From each group, 20 animals were not sacrificed after AP for a 7-day mortality study. Serum cytokine levels did not differ among groups at any time point, but the intragroup analysis over time showed higher interferon-gamma only in the nontreatment and saline groups at 2 h (versus 12 and 24 h; both p <= 0.05). The glutamine group exhibited greater lung and liver HSP90 expression than did the nontreatment group at 2 and 12 h, respectively; greater liver HSP90 and HSP70 expression than did the saline group at 12 h; and smaller lung HSP70 and liver HSP90 expression than did the nontreatment group at 24 h (all p <= 0.019). The 7-day mortality rate did not differ among groups. In experimental AP, pretreatment with parenteral glutamine was safe and improved early inflammatory mediator profiles without affecting mortality. (AU)

• FAPESP's process: 11/02071-7 - Impact of prior use of parenteral omega-3 fatty acids and glutamine isolated and combined under the systemic inflammatory response and mortality in experimental acute pancreatitis
• Grantee: Dan Linetzky Waitzberg
• Support Opportunities: Regular Research Grants