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Analysis of lipid mediators prostaglandin PGE2, TXB2 thromboxane, leukotrienes LTB4, lipoxin A4 and resolvin E1 in rat lung after parenteral infusion of omega-3 lipid emulsion after 12 and 24 hours of induction of severe acute pancreatitis

Grant number: 13/25796-2
Support type:Scholarships abroad - Research Internship - Master's degree
Effective date (Start): May 19, 2014
Effective date (End): October 30, 2014
Field of knowledge:Health Sciences - Nutrition
Principal Investigator:Dan Linetzky Waitzberg
Grantee:Priscila Casarin Garla
Supervisor abroad: Philip Calder
Home Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Local de pesquisa : University of Southampton, England  
Associated to the scholarship:12/13691-9 - Effect of prior parenteral use of omega-3 and glutamine isolated and combined submitted the systemic inflammatory response and mortality in experimental acute pancreatitis, BP.MS


Current evidence supports that n-3 PUFA, particularly EPA and DHA found in fish oil, can attenuate the development of inflammatory diseases by affecting different steps of the immune response. Their capacity to modulate eicosanoid synthesis, nuclear receptor and nuclear transcription factors activity and resolvin production may mitigate inflammatory processes already present, contributing to treatment of pathological inflammation. However, our preliminary results have shown that the infusion of omega-3 PUFA, in rats submitted to severe acute pancreatitis (AP), was associated with increased plasma pro-inflammatory (IL-1 and IL-2) and decreased anti-inflammatory (IL-10) cytokine levels after 12 hours of AP. This treatment was also associated to the reduction of HSP 90 in liver tissue, after 2 hours of induction of AP and a greater weight loss. Our results are distinct from the beneficial findings observed in previous experimental and clinical trials assessing the use of parenteral omega-3 PUFAs in AP 22-26. More detailed analysis focusing on other immune and inflammatory response markers in lung may significantly contribute to better understanding of our results. (AU)