Impact of hormonal modulation at proestrus on ovarian responses and uterine gene expression of suckled anestrous beef cows

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de Sa Filho, Manoel Francisco ; Gonella-Diaza, Angela Maria ; Sponchiado, Mariana ; Mendanha, Marcio Ferreira ; Pugliesi, Guilherme ; Ramos, Roney dos Santos ; da Silva Andrade, Sonia Cristina ; Gasparin, Gustavo ; Coutinho, Luiz Lehmann ; Goissis, Marcelo Demarchi ; Mesquita, Fernando Silveira ; Baruselli, Pietro Sampaio ; Binelli, Mario

• Document type: Journal article
• Source: JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY; v. 8, p. 14-pg., 2017-11-01.

Abstract

Background: This study evaluated the impact of hormonal modulation at the onset of proestrus on ovarian response and uterine gene expression of beef cows. Methods: A total of 172 anestrous beef cows were assigned to one of four groups according to the treatment with estradiol cypionate (ECP) and/or equine chorionic gonadotropin (eCG) [CON (n = 43), ECP (n = 43), eCG (n = 44) and ECP + eCG (n = 42)]. Results: ECP-treated cows (ECP and ECP + eCG groups) presented greater occurrence of estrus (44.6% vs. 65.4%; P = 0. 01) and pregnancy per AI [47.1% vs. 33.3%; P = 0.07], but similar progesterone (P4) concentration at subsequent diestrus than cows not treated with ECP (CON and eCG groups). Nonetheless, eCG-treated cows (eCG and ECP + eCG groups) presented larger follicle at timed AI (12.6 +/- 0.3 vs. 13.5 +/- 0.3 mm; P = 0.03), greater ovulation rate (96.5% vs. 82.6%; P = 0.008) and greater P4 concentration at d 6 (3.9 +/- 0.2 vs. 4.8 +/- 0.2 ng/mL; P = 0.001) than cows not treated with eCG (CON and ECP groups). Next, cows with a new corpus luteum 6 d after TAI were submitted to uterine biopsy procedure. Uterine fragments [CON (n = 6), ECP (n = 6)] were analyzed by RNA-Seq and a total of 135 transcripts were differentially expressed between groups (73 genes up-regulated by ECP treatment). Subsequently, uterine samples were analyzed by qPCR (genes associated with cell proliferation). ECP treatment induced greater abundance of PTCH2 (P = 0.07) and COL4A1 (P = 0.02), whereas suppressed EGFR (P = 0.09) expression. Conversely, eCG treatment increased abundance of HB-EGF (P = 0.06), ESR2 (P = 0.09), and ITGB3 (P = 0.05), whereas it reduced transcription of ESR1 (P = 0.05). Collectively, supplementation with ECP or eCG at the onset of proestrous of anestrous beef cows influenced ovarian responses, global and specific endometrial gene expression. Conclusion: Proestrus estradiol regulate the endometrial transcriptome, particularly stimulating proliferative activity in the endometrium. (AU)

• FAPESP's process: 12/14731-4 - Different hormone stimulus during pro-estrous after synchronization of ovulation programs on uterine receptivity for gestation of suckled zebu females.
• Grantee: Manoel Francisco de Sá Filho
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 11/03226-4 - Signatures of receptivity
• Grantee: Mario Binelli
• Support Opportunities: Research Projects - Thematic Grants