Photobiomodulation Leads to Reduced Oxidative Stress in Rats Submitted to High-Intensity Resistive Exercise

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de Oliveira, Helenita Antonia ; Antonio, Ednei Luiz ; Arsa, Gisela ; Santana, Eduardo Tadeu ; Silva, Flavio Andre ; Arruda Junior, Daniel ; dos Santos, Simone ; Camillo de Carvalho, Paulo de Tarso ; Pinto Leal-Junior, Ernesto Cesar ; Araujo, Amanda ; De Angelis, Katia ; Bocalini, Danilo Sales ; Silva Junior, Jose Antonio ; Ferreira Tucci, Paulo Jose ; Serra, Andrey Jorge

• Document type: Journal article
• Source: OXIDATIVE MEDICINE AND CELLULAR LONGEVITY; v. 2018, p. 9-pg., 2018-01-01.

Abstract

The aim of this study was to determine whether oxidative stress markers are influenced by low-intensity laser therapy (LLLT) in rats subjected to a high-intensity resistive exercise session (RE). Female Wistar rats divided into three experimental groups (Ctr: control, 4J: LLLT, and RE) and subdivided based on the sampling times (instantly or 24 h postexercise) underwent irradiation with LLLT using three-point transcutaneous method on the hind legs, which was applied to the gastrocnemius muscle at the distal, medial, and proximal points. Laser (4J) or placebo (device off) were carried out 60 sec prior to RE that consisted of four climbs bearing the maximum load with a 2 min time interval between each climb. Lipoperoxidation levels and antioxidant capacity were obtained in muscle. Lipoperoxidation levels were increased (4-HNE and CL markers) instantly post-RE. LLLT prior to RE avoided the increase of the lipid peroxidation levels. Similar results were also notified for oxidation protein assays. The GPx and FRAP activities did not reduce instantly or 24 h after RE. SOD increased 24 h after RE, while CAT activity did not change with RE or LLLT. In conclusion, LLLT prior to RE reduced the oxidative stress markers, as well as, avoided reduction, and still increased the antioxidant capacity. (AU)

• FAPESP's process: 17/09274-7 - Functional and proteomic characterization of cardiomyocytes and stem cells submitted to physiological and pathological stimulus
• Grantee: Andrey Jorge Serra
• Support Opportunities: Research Grants - Visiting Researcher Grant - International
• FAPESP's process: 15/11028-9 - The aerobic training method can determine the existence of cardiac remodeling: structural, functional and molecular analyzes of swimming and running experimental models.
• Grantee: Andrey Jorge Serra
• Support Opportunities: Regular Research Grants