Antitumor Potential of S-Nitrosothiol-Containing Polymeric Nanoparticles against Melanoma

Author(s)

Ferraz, Leticia S. ; Watashi, Carolina M. ; Colturato-Kido, Carina ; Pelegrino, Milena T. ; Paredes-Gamero, Edgar J. ; Weller, Richard B. ; Seabra, Amedea B. ; Rodrigues, Tiago

• Document type: Journal article
• Source: MOLECULAR PHARMACEUTICS; v. 15, n. 3, p. 9-pg., 2018-03-01.

Abstract

Melanoma is a malignant proliferative disease originated from melanocyte transformations, which are characterized by a high metastatic rate and mortality. Advances in Nanotechnology have provided useful new approaches and tools for antitumor chemotherapy. The aim of this study was to investigate the molecular mechanisms underlying chitosan nanoparticles containing S-nitrosomercaptosuccinic acid (S-nitroso-MSA-CS) induced cytotoxicity in melanoma cells. S-Nitroso-MSA-CS induced concentration dependent cell death against B16-F10 tumor cells, whereas non-nitroso nanopartides (CS or MSA-CS) did not induce significant cytotoxicity. Additionally, melanoma cells were more sensitive to cell death than normal melanocytes. S-Nitroso-MSA-CS-induced cytotoxicity exhibited features of caspase-dependent apoptosis, and it was associated with oxidative stress, characterized by increased mitochondrial superoxide production and oxidation of protein thiol groups. In addition, tyrosine nitration and cysteine S-nitrosylation of amino acid residues in cellular proteins were observed. The potential use of these nanoparticles in antitumor chemotherapy of melanoma is discussed. (AU)

• FAPESP's process: 16/07367-5 - Investigation of Phenothiazine-Induced Cell Death Mechanisms In Tumor Cells: Changes in Gene Expression, Role of Bcl-2 Family Proteins, and ER Stress
• Grantee: Tiago Rodrigues
• Support Opportunities: Regular Research Grants
• FAPESP's process: 12/12247-8 - New applications of phenothiazines and Palladacycles: nanostructured systems to the mechanistic study of death in tumor cells
• Grantee: Tiago Rodrigues
• Support Opportunities: Regular Research Grants