| Full text | |
| Author(s): |
de Carvalho, Francine Pereira
;
Moretto, Thais Ludmilla
;
Benfato, Izabelle Dias
;
Barthichoto, Marcela
;
Ferreira, Sandra Mara
;
Costa-Junior, Jose Maria
;
Machado de Oliveira, Camila Aparecida
Total Authors: 7
|
| Document type: | Journal article |
| Source: | BIOSCIENCE REPORTS; v. 38, p. 12-pg., 2018-04-27. |
| Abstract | |
To investigate the central (hypothalamic) and peripheral effects of exercise without body weight change in diet-induced obesity (DIO). Twelve-week-old male C57BI/6 mice received a control (C) or a high-fat diet (H). Half of them had free access to running wheels for 5 days/week for 10 weeks (CE) and HE, respectively). Hypothalamic expression of genes related to energy homeostasis, and leptin (Stat3 and p-Stat3) and insulin (Akt and p-Akt) signaling were evaluated. Glucose and leptin tolerance, peripheral insulin sensitivity, and plasma insulin, leptin and adiponectin were determined. Perigonadal and retroperitoneal fat depots were increased by diet but reduced by exercise despite lack of effect of exercise on body weight. Blood glucose during intraperitoneal glucose tolerance test (ipGTT) was higher and glucose decay during intraperitoneal insulin tolerance test (ipITT) was lower in H and HE compared with C and CE. Exercise increased liver p-Akt expression and reduced fast glycemia. High-fat diet increased plasma insulin and leptin. Exercise had no effect on insulin but decreased leptin and increased adiponectin. Leptin inhibited food intake in all groups. Hypothalamic total and p-Stat3 and Akt were similar amongst the groups despite higher plasma levels of leptin and insulin in H and HE mice. High-fat diet modulated gene expression favoring a positive energy balance. Exercise only marginally changed the gene expression. Exercise induced positive changes (decreased fast glycemia and fat depots; increased liver insulin signaling and adiponectin concentration) without weight loss. Thus, despite reducing body weight could bring additional benefits, the effects of exercise must not be overlooked when weight reduction is not achieved. (AU) | |
| FAPESP's process: | 13/01624-8 - Effects of different diets (low protein and high fat diet) on spontaneous physical activity and hypothalamic expression of genes related to energy homeostasis in mice |
| Grantee: | Thais Ludmilla Moretto |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 11/05932-3 - Molecular mechanisms involved in the modulation of spontaneous physical activity and development of obesity |
| Grantee: | Camila Aparecida Machado de Oliveira |
| Support Opportunities: | Research Grants - Young Investigators Grants |