Effects of Diacetyl Flavoring Exposure in Mice Metabolism

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Lima Jedlicka, Leticia Dias ; Silva, Juciara da Costa ; Balbino, Aleksandro Martins ; Neto, Giuseppe Bruno ; Sousa Furtado, Danielle Zildeana ; Torres da Silva, Heron Dominguez ; Correia Cavalcanti, Fernandade Barros ; van der Heijden, Karin Marie ; Avellaneda Penatti, Carlos Alberto ; Henriques Bechara, Etelvino Jose ; Assuncao, Nilson Antonio

• Document type: Journal article
• Source: BIOMED RESEARCH INTERNATIONAL; v. 2018, p. 11-pg., 2018-01-01.

Abstract

Diacetyl is a flavoring that imparts a buttery flavor to foods, but the use or exposure to diacetyl has been related to some diseases. We investigated the effect of oral intake of diacetyl in male and female C57/Bl mice. We performed a target metabolomics assay using ultraperformance liquid chromatography paired with triple quadrupole mass spectrometry (UPLC-MS/MS) for the determination and quantification of plasmatic metabolites. We observed alterations in metabolites present in the urea and tricarboxylic acid (TCA) cycles. Peroxynitrite plasmatic levels were evaluated by a colorimetric method, final activity of superoxide dismutase (SOD) was evaluated by an enzymatic method, and mouse behavior was evaluated. Majority of the assay showed differences between control and treatment groups, as well as between genders. This may indicate the involvement of sex hormones in the regulation of a normal metabolic profile, and the implication of sex differences in metabolite disease response. (AU)

• FAPESP's process: 13/07763-0 - Studies of Radical Acetylation of Proteins Using Proteomics Tools
• Grantee: Leticia Dias Lima Jedlicka
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 18/02385-0 - Correlation between the Metabolic, Lipidomic and Proteomic Profiles and the 5p Deletion Size of Cri-Du-Chat Syndrome Patients
• Grantee: Nilson Antonio de Assunção
• Support Opportunities: Regular Research Grants
• FAPESP's process: 12/02514-9 - Use of proteomics techniques to study radical acetylation of proteins triggered by products of the reaction biacetyl/peroxinitrite
• Grantee: Nilson Antonio de Assunção
• Support Opportunities: Regular Research Grants