Differential Expression of Prostaglandin I2 Synthase Associated with Arachidonic Acid Pathway in the Oral Squamous Cell Carcinoma

Author(s)

Russo, Anelise ; Biselli-Chicote, Patricia M. ; Kawasaki-Oyama, Rosa S. ; Castanhole-Nunes, Marcia M. U. ; Maniglia, Jose V. ; Neto, Dalisio de Santi ; Pavarino, Erika C. ; Goloni-Bertollo, Eny M.

• Document type: Journal article
• Source: JOURNAL OF ONCOLOGY; v. 2018, p. 13-pg., 2018-01-01.

Abstract

Introduction. Differential expression of genes encoding cytochrome P450 (CYP) and other oxygenases enzymes involved in biotransformation mechanisms of endogenous and exogenous compounds can lead to oral tumor development. Objective. We aimed to identify the expression profile of these genes, searching for susceptibility biomarkers in oral squamous cell carcinoma. Patients and Methods. Sixteen oral squamous cell carcinoma samples were included in this study (eight tumor and eight adjacent non-tumor tissues). Gene expression quantification was performed using TaqMan Array Human CYP450 and other Oxygenases 96-well plate (Applied Biosystems) by real time qPCR. Protein quantification was performed by ELISA and IHC methods. Bioinformatics toolswere used to findmetabolic pathways related to the enzymes encoded by differentially expressed genes. Results. CYP27B1, CYP27A1, CYP2E1, CYP2R1, CYP2J2, CYP2U1, CYP4F12, CYP4X1, CYP4B1, PTGIS, ALOX12, and MAOB genes presented differential expression in the oral tumors. After correction by multiple tests, only the PTGIS (Prostaglandin I2 Synthase) gene presented significant differential expression (P < 0.05). The PTGIS gene and protein were reduced in oral tumors. Conclusion. PTGIS presents downexpression in oral tumors. PTGIS play an important role in the arachidonic acid metabolism. Arachidonic acid and/or metabolites are derived from this pathway, which can influence the regulation of important physiological mechanisms in tumorigenesis process. (AU)

• FAPESP's process: 13/04923-6 - Expression of genes involved in xenobiotic metabolism pathway in patients with head and neck cancer
• Grantee: Eny Maria Goloni Bertollo
• Support Opportunities: Regular Research Grants