Reference genes for gene expression studies targeting sugarcane infected with Sugarcane mosaic virus (SCMV)

Author(s)

da Silva, Marcel Fernando ; Goncalves, Marcos Cesar ; Brito, Michael dos Santos ; Nobile, Paula Macedo ; de Andrade, Larissa Mara ; Medeiros, Cibele Nataliane ; Creste, Silvana ; Pinto, Luciana Rossini

• Document type: Journal article
• Source: BMC RESEARCH NOTES; v. 12, p. 7-pg., 2019-03-18.

Abstract

Objective: The selection of reference genes in sugarcane under Sugarcane mosaic virus (SCMV) infection has not been reported and is indispensable to get reliable reverse transcription quantitative PCR (RT-qPCR) results for validation of transcriptome analysis. In this regard, seven potential reference genes were tested by RT-qPCR and ranked according to their stability using BestKeeper, NormFinder and GeNorm algorithms, and RefFinder WEB-based software in an experiment performed with samples from two sugarcane cultivars contrasting for SCMV resistance, when mechanically inoculated with a severe SCMV strain and using mock inoculated plant controls. Results: The genes Uridylate kinase (UK) and Ubiquitin-conjugating enzyme 18 (UBC18) were the most stable according to GeNorm algorithm and the Pearson correlation coefficients with the BestKeeper index. On the other hand, ribosomal protein L35-4 (RPL1), Actin (ACT) and Ubiquitin1 (UBQ1) were the least stable genes for all algorithms tested. (AU)

• FAPESP's process: 12/15060-6 - Identification of candidate genes for sugarcane mosaic virus through cDNA-AFLP.
• Grantee: Cibele Nataliane Facioli Medeiros
• Support Opportunities: Scholarships in Brazil - Master