Identification of candidate genes for sugarcane mosaic virus through cDNA-AFLP.

• Grant number: 12/15060-6
• Support type: Scholarships in Brazil - Master
• Effective date (Start): October 01, 2012
• Effective date (End): July 31, 2013
• Field of knowledge: Agronomical Sciences - Agronomy - Crop Science
• Principal Investigator: Luciana Rossini Pinto Machado da Silva
• Grantee: Cibele Nataliane Facioli Medeiros
• Home Institution: Instituto Agronômico (IAC). Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). Campinas , SP, Brazil

Abstract

Sugarcane mosaic disease caused by the Sugarcane Mosaic Virus (SCMV) is one of the main sugarcane viruses. The use of resistant cultivars is the most efficient mean of control. The cDNA-AFLP approach has being used to detect genetic polymorphism between contrast phenotypes under controlled conditions. Such polymorphisms can be used to the identification of candidate genes and also to marker development for genetic mapping. The objective of the present work was to identify potential candidate genes related to mosaic resistance using the cDNA-AFLP technique. Two sugarcane cultivars IACSP95-5000 (resistant) and IAC91-1099 (susceptible) obtained through tissue culture will be inoculated with a strong strain of the virus (Rib-1) at green-house condition with three biological replicates for each sample times (0, 24, 48 and 72 hours after inoculation) and respective controls (non-inoculated plants). After inoculation the leaves of each cultivar will be collected at each sample time to the identification of the differentiated expressed fragments revealed through cDNA-AFLP. These fragments, after cloning and sequencing for homology identification could be applied to the development of a primer set with potential use in genetic mapping of sugarcane mosaic resistance regions.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA SILVA, MARCEL FERNANDO; GONCALVES, MARCOS CESAR; BRITO, MICHAEL DOS SANTOS; MEDEIROS, CIBELE NATALIANE; HARAKAVA, RICARDO; DE ANDRADE LANDELL, MARCOS GUIMARAES; PINTO, LUCIANA ROSSINI. Sugarcane mosaic virus mediated changes in cytosine methylation pattern and differentially transcribed fragments in resistance-contrasting sugarcane genotypes. PLoS One, v. 15, n. 11 NOV 9 2020. Web of Science Citations: 0.