| Full text | |
| Author(s): |
Bernardi, Aline Vianna
;
Yonamine, Deborah Kimie
;
Uyemura, Sergio Akira
;
Dinamarco, Taisa Magnani
Total Authors: 4
|
| Document type: | Journal article |
| Source: | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 20, n. 9, p. 15-pg., 2019-05-01. |
| Abstract | |
In the context of avoiding the use of non-renewable energy sources, employing lignocellulosic biomass for ethanol production remains a challenge. Cellulases play an important role in this scenario: they are some of the most important industrial enzymes that can hydrolyze lignocellulose. This study aims to improve on the characterization of a thermostable Aspergillus fumigatus endo-1,4--glucanase GH7 (Af-EGL7). To this end, Af-EGL7 was successfully expressed in Pichia pastoris X-33. The kinetic parameters K-m and V-max were estimated and suggested a robust enzyme. The recombinant protein was highly stable within an extreme pH range (3.0-8.0) and was highly thermostable at 55 degrees C for 72 h. Low Cu2+ concentrations (0.1-1.0 mM) stimulated Af-EGL7 activity up to 117%. Af-EGL7 was tolerant to inhibition by products, such as glucose and cellobiose. Glucose at 50 mM did not inhibit Af-EGL7 activity, whereas 50 mM cellobiose inhibited Af-EGL7 activity by just 35%. Additionally, the Celluclast((R)) 1.5L cocktail supplemented with Af-EGL7 provided improved hydrolysis of sugarcane bagasse in natura, sugarcane exploded bagasse (SEB), corncob, rice straw, and bean straw. In conclusion, the novel characterization of Af-EGL7 conducted in this study highlights the extraordinary properties that make Af-EGL7 a promising candidate for industrial applications. (AU) | |
| FAPESP's process: | 18/11231-7 - Functional characterization of GH7 endoglucanase from Aspergillus fumigatus |
| Grantee: | Deborah Kimie Yonamine |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 14/10466-0 - Transcriptome analysis of Aspergillus fumigatus grown on sugarcane bagasse |
| Grantee: | Taisa Magnani Dinamarco |
| Support Opportunities: | Program for Research on Bioenergy (BIOEN) - Regular Program Grants |