Long-term increase of insulin secretion in mice subjected to pregnancy and lactation

Author(s):Show less -

Vicente, Julia Modesto ; Santos-Silva, Junia Carolina ; Teixeira, Caio Jordao ; de Souza, Dailson Nogueira ; Vettorazzi, Jean Franciesco ; Furtuoso, Fabiola Sales ; Adabo, Isabel Gouveia ; Sato, Fabio Takeo ; Ramirez Vinolo, Marco Aurelio ; Carneiro, Everardo Magalhaes ; Bordin, Silvana ; Anhe, Gabriel Forato

• Document type: Journal article
• Source: ENDOCRINE CONNECTIONS; v. 9, n. 4, p. 10-pg., 2020-04-01.

Abstract

Purpose: Observational studies show that longer breastfeeding periods reduce maternal risk of type 2 diabetes mellitus. However, it is currently unknown if the long-term benefits of breastfeeding for maternal glucose homeostasis are linked to changes in the endocrine pancreas. Methods: We presently evaluated functional, morphological and molecular aspects of the endocrine pancreas of mice subjected to two sequential cycles of pregnancy and lactation (L21). Age-matched mice not allowed to breastfeed (LO) and virgin mice were used as controls. Results: L21 mice exhibited increased tolerance and increased glucose-stimulated insulin secretion (GSIS) by isolated islets. Pancreatic islets of L21 mice did not present evident morphological changes to justify the increased GSIS. On the other hand, islets of L21 mice exhibited a reduction in Cavb3 and Kir6.2 expression with concordant increased intracellular Ca2+ levels after challenge with glucose. Conclusion: Altogether, the present findings show the breastfeeding exerts long-term benefits for maternal endocrine pancreas by increasing intracellular Ca2+ levels and GSIS. (AU)

• FAPESP's process: 15/12680-1 - Lon-term impacts of breastfeeding on maternal metabolism: Participation of microRNAs
• Grantee: Gabriel Forato Anhê
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/07607-8 - OCRC - Obesity and Comorbidities Research Center
• Grantee: Licio Augusto Velloso
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 17/11138-4 - Effect of pregnancy followed by lactation on miRNAs modulation of maternal pancreatic islets
• Grantee: Julia Modesto Vicente
• Support Opportunities: Scholarships in Brazil - Scientific Initiation