Decrease of miR-125a-5p in Gastritis and Gastric Cancer and Its Possible Association with H. pylori

Author(s)

dos Santos, Monica Pezenatto ; Pereira, Jessica Nunes ; De Labio, Roger Willian ; Carneiro, Lilian Carla ; Pontes, Jaqueline Correia ; Barbosa, Monica Santiago ; Cardoso Smith, Marilia De Arruda ; Marques Payao, Spencer Luiz ; Rasmussen, Lucas Trevizani

• Document type: Journal article
• Source: JOURNAL OF GASTROINTESTINAL CANCER; v. 52, n. 2, p. 6-pg., 2020-06-06.

Abstract

Purpose The aim of this study was to evaluate the expression of miR-125a-5p in patients with dyspeptic symptoms and gastric cancer, correlating them with the development of this cancer and H. pylori. Methods Patients were divided in groups according to histopathological analysis (control, gastritis, and cancer groups). Polymerase chain reaction was performed to detect H. pylori and real-time quantitative PCR to determine miR-125a-5p expression. Results H. pylori was detected in 44% of the patients, with prevalence in the gastritis and cancer groups. A statistically significant decrease of miR-125a-5p expression was found in the control positive (p = 0.0183*), gastritis positive (p = 0.0380*), and cancer positive (p = 0.0288*) groups when compared with the control negative group. Conclusion We suggest that decreased expression of the miRNA-125a-5p associated with the presence of the H. pylori is an important mechanism in gastric diseases and could be a possible marker for early diagnosis of gastric cancer. (AU)

• FAPESP's process: 18/02008-2 - Analysis of microRNA expression miR-let7a, miR-125a, miR-155 and the genes of interleukin 6 and its receptor-alpha in patients with gastric diseases and in cell culture: a possible association with H. pylori
• Grantee: Monica Pezenatto dos Santos
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 18/08481-1 - Gene and Protein Interaction among Components of the Inflammatory, Apoptosis and microRNAs Pathways in Gastric Disease and Gastric Cancer
• Grantee: Lucas Trevizani Rasmussen
• Support Opportunities: Regular Research Grants