Dysregulated Expression of Apoptosis-Associated Genes and MicroRNAs and Their Involvement in Gastric Carcinogenesis

Author(s)

Sallas, Mayara Luciana ; Zapparoli, Diana ; dos Santos, Monica Pezenatto ; Pereira, Jessica Nunes ; Orcini, Wilson Aparecido ; Peruquetti, Rita Luiza ; Chen, Elizabeth Suchi ; de Arruda Cardoso Smith, Marilia ; Payao, Spencer Luiz Marques ; Rasmussen, Lucas Trevizani

• Document type: Journal article
• Source: JOURNAL OF GASTROINTESTINAL CANCER; v. 52, n. 2, p. 9-pg., 2020-06-24.

Abstract

Purpose Analyze the expression of caspase-9, Smac/DIABLO, XIAP, let-7a, and let-7b in patients with normal gastric tissue, chronic gastritis, and gastric adenocarcinoma. Methods The expression ofcaspase-9,Smac/DIABLO,XIAP, let-7a, and let-7b by qRT-PCR was analyzed in 158 samples from 53 patients with normal gastric mucosa, 86 with chronic gastritis, and 19 with gastric cancer. Results The comparison between the gastric cancer and the control group revealed a decreased expression ofcaspase-9in gastric cancer tissues; considering theHelicobacter pylorpresence, comparable results were revealed.Smac/DIABLOwas increased in gastric cancer cells, whileXIAPdemonstrated no significant difference in the gene expression. The microRNA analysis revealed a decreased expression of let-7a and let-7b in samples positive toH. pyloriinfection and in gastric cancer group, regardless of the presence of the bacterium. Conclusion Our study provided some evidence of low activity of the intrinsic apoptosis pathway, as well as the influence ofH. pylorion let-7a and let-7b expression. (AU)

• FAPESP's process: 15/11371-5 - Analysis of Expression of Micro RNAs and Genes Associated with Apoptosis and Chronic Inflammation in Gastric Diseases: A Possible Relation with Helicobacter pylori
• Grantee: Lucas Trevizani Rasmussen
• Support Opportunities: Regular Research Grants