Regulatory T cells isolated from endometriotic peritoneal fluid express a different number of Toll-like receptors

Author(s)

Hernandes, Camila ; Gueuvoghlanian-Silva, Barbara Yasmin ; Monnaka, Vitor Ulisses ; Ribeiro, Natalia Mazini ; Pereira, Welbert de Oliveira ; Podgaec, Sergio

• Document type: Journal article
• Source: Einstein (São Paulo); v. 18, p. 6-pg., 2020-01-01.

Abstract

Objective: To analyze and compare the expression of Toll-like receptors by regulatory T cells present in the peritoneal fluid of patients with and without endometriosis. Methods: Regulatory T cells were isolated from peritoneal fluid of women with and without endometriosis, collected during surgery, and mRNA was extracted for analysis of Toll-like receptors expression by reverse-transcriptase polymerase chain reaction. Results: Patients with endometriosis presented regulatory T cells expressing a larger number and variety of Toll-like receptors when compared to regulatory T cells from patients in the Control Group. Toll-like receptor-1 and Toll-like receptor-2 in regulatory T cells were expressed in both groups. All other expressed Toll-like receptors types were only found in regulatory T cells from the Endometriosis Group. Conclusion: Patients with endometriosis had peritoneal regulatory T cells expressing various Toll-like receptors types. (AU)

• FAPESP's process: 14/08227-7 - Regulatory T cells activation in deep endometriosis
• Grantee: Bárbara Yasmin Gueuvoghlanian Silva Lopes
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 13/27092-2 - Regulatory T cells activation in deep endometriosis
• Grantee: Sérgio Podgaec
• Support Opportunities: Regular Research Grants