Evaluation of pulsed electromagnetic field protocols in implant osseointegration: in vivo and in vitro study

Author(s)

Nunes, Camilla Magnoni Moretto ; Ferreira, Camila Lopes ; Bernardo, Daniella Vicensotto ; Lopes, Cassia Carolina Rabelo ; Collino, Luma ; da Silva Lima, Victoria Clara ; de Camargo Reis Mello, Daphne ; de Vasconcellos, Luana Marotta Reis ; Jardini, Maria Aparecida Neves

• Document type: Journal article
• Source: CLINICAL ORAL INVESTIGATIONS; v. 25, n. 5, p. 13-pg., 2020-10-09.

Abstract

Objectives The present study aims to evaluate two protocols of pulsed electromagnetic field (PEMF) on osseointegration and establish one that addresses ideal parameters for its use in dentistry, especially in the optimization of the implants osseointegration process. Materials and methods Sixty male rats (Wistar) were allocated into three experimental groups: control (GC), test A (GTA, 3 h exposed), and test B (GTB, 1 h exposed). All animals received titanium implants in both tibias, and PEMF application (15 Hz, +/- 1 mT, 5 days/week) occurred only in the test groups. They were euthanized at 03, 07, 21, and 45 days after PEMF therapy. Removal torque, histomorphometric measurements, three-dimensional radiographic evaluation, and in vitro biological assay analyses were performed. Results GTB showed better results compared with GTA in removal torque tests, in bone volume and bone mineral density, cell viability, total protein content, and mineralization nodules (p < 0.05). GTA showed better performance in trabecular bone thickness and cell proliferation compared with GTB (p < 0.05), especially at osseointegration early periods. In the histomorphometric analysis and number of trabeculae, there were no differences in the test groups. Conclusion PEMF as a biostimulator was effective in optimizing the events in bone tissue that lead to osseointegration, especially when applied for a shorter time and in the initial periods of bone healing. (AU)

• FAPESP's process: 14/50983-3 - INCT 2014: complex fluids
• Grantee: Antonio Martins Figueiredo Neto
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/07340-5 - Structural characterisation of lipoproteins
• Grantee: Zahra Lotfollahi
• Support Opportunities: Scholarships in Brazil - Post-Doctoral