Antimicrobial photodynamic therapy reduces gene expression of Candida albicans in biofilms

Author(s)

Jordao, Claudia Carolina ; de Sousa, Tabata Viana ; Klein, Marlise Inez ; Dias, Luana Mendonca ; Pavarina, Ana Claudia ; Carmello, Juliana Cabrini

• Document type: Journal article
• Source: Photodiagnosis and Photodynamic Therapy; v. 31, p. 7-pg., 2020-09-01.

Abstract

The present study evaluated whether the oxidative stress caused by antimicrobial photodynamic therapy (aPDT) affects the expression of C. albicans genes related to adhesion and biofilm formation (ALS1 and HPW1) and oxidative stress response (CAP1, CAT1, and SOD1). The aPDT was mediated by two photosensitizing agents (PSs) Photodithazine (R) (PDZ at 100 and 200 mg/L) or Curcumin (CUR at 40 and 80 mu M) and LED (37.5 J/cm(2) or 50 J/cm(2)). The quantification of the expression was performed by Reverse Transcription-Quantitative Polymerase Chain Reaction (RT-qPCR) using specific primers for the target genes. The data were analyzed by Analysis of Variance (alpha = 0.05), followed by Tukey's post-test. It was observed reduction in the expression of ALS1, HWP1, CAP1, CAT1, and SOD1 when aPDT was performed using 200 mg/L PDZ and 80 mu M CUR associated to LED (37.7 and 50 J/cm(2), respectively) and using 100 mg/L PDZ and 40 mu M CUR with LED of 50 J/cm(2) (versus control). Also, the expression of CAP1 and SOD1 genes was reduced after aPDT using 100 mg/L PDZ and LED of 37.5 J/cm(2). There was a significant reduction in the expression of genes HWP1, CAP1, and SOD1 after aPDT using 40 mu M CUR and 37.5 J/cm(2) (versus the control group). The application of LED only at 37.5 and 50 J/cm(2) promoted down-regulation of ALS1, CAP1, CAT1, and SOD1 genes (versus the control group). Therefore, aPDT mediated by LED -associated PSs PDZ and CUR promoted a reduction in the expression of the five C. albicans genes evaluated. (AU)

• FAPESP's process: 16/13868-7 - Evaluation of the fototoxic and fotobiomodulador effects of different wavelengths of Light Emitting Diode (led) radiation on human cells and micro-organims
• Grantee: Juliana Cabrini Carmello
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 17/12358-8 - Evaluation of gene expression related to the virulence factors and response to the oxidative stress of biofilms of Candida albicans submitted to antimicrobial photodynamic therapy mediated by Photoditazine® and curcumin
• Grantee: Tábata Viana de Sousa
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 13/07276-1 - CEPOF - Optics and Photonic Research Center
• Grantee: Vanderlei Salvador Bagnato
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC