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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Impaired Bacillus Calmette-Guerin cellular immune response in HIV-exposed, uninfected infants

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Author(s):
Mazzola, Tais N. [1] ; da Silva, Marcos T. N. [1] ; Abramczuk, Beatriz M. [1] ; Moreno, Yara M. F. [1, 2] ; Lima, Simone C. B. S. [1] ; Zorzeto, Tatiane Q. [1] ; Passeto, Andrea S. Z. [1] ; Vilela, Maria M. S. [1]
Total Authors: 8
Affiliation:
[1] State Univ Campinas Med Sch UNICAMP, Dept Pediat, Ctr Pediat Invest CIPED, BR-13083887 Sao Paulo - Brazil
[2] Univ Fed Santa Catarina, Dept Nutr, Hlth Sci Ctr, Florianopolis, SC - Brazil
Total Affiliations: 2
Document type: Journal article
Source: AIDS; v. 25, n. 17, p. 2079-2087, NOV 13 2011.
Web of Science Citations: 32
Abstract

Objective: To evaluate cell-mediated immune response to Bacillus Calmette-Guerin (BCG) vaccination in uninfected, HIV-1-exposed infants, comparing it with unexposed children. Design: It is designed as a cross-sectional study. Methods: BCG-specific lymphoproliferation and T-cell subsets (CD4(+), CD8(+) and TCR gamma delta(+)) by flow cytometry and interleukin-10, interferon-gamma (IFN-gamma) and tumor necrosis factor-alpha (TNF-alpha) concentration by ELISA were analyzed in HIV-exposed and unexposed infants. Whole blood lymphocyte immunophenotyping and blood counts were performed in exposed children. Nonparametric tests were used (P < 0.05). Results: Given the ontogeny of the immune system, exposed infants were separated into three groups according to age: exposed 1 (E1, aged 6.1-8.8 months), E2 (aged 9.1-17.1 months) and E3 (aged 18.1-26.3 months). Unexposed infants (UE group) and E1 were matched for age. Cell proliferation was not different among the three exposed groups, neither for BCG nor for phytohemagglutinin (PHA)-stimulated cultures. Furthermore, BCG-stimulated lymphoproliferation was reduced in the E1 group in comparison with the UE group. T-lymphocyte subpopulations also showed differences, with the youngest HIV-exposed groups (E1 and E2) showing a predominant proliferation of CD4(+) T cells in cultures with BCG, whereas E3 and UE groups had a robust gamma delta(+) T-cell expansion. There was lower IFN-gamma concentration in the samples from E1 group in comparison with all of the other groups. The unexposed infants showed higher TNF-alpha concentration in cultures with BCG and PHA in comparison with E1 group. Conclusion: BCG-specific T-cell proliferation was reduced in HIV-exposed uninfected infants and IFN-gamma concentration was lower in younger exposed infants, showing a delay in immune system maturation of HIV-exposed infants. (C) 2011 Wolters Kluwer Health vertical bar Lippincott Williams \& Wilkins (AU)

FAPESP's process: 05/02277-3 - Nutritional status and immunity for BCG and Hepatitis B vaccines in children with AIDS under nutritional supplementation with prebiotics
Grantee:Maria Marluce dos Santos Vilela
Support Opportunities: Regular Research Grants