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Evaluation of Elafin Immunohistochemical Expression as Marker of Cervical Cancer Severity

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Author(s):
Longatto-Filho, Adhemar ; Fregnani, Jose Humberto ; da Costa, Allini Mafra ; de Araujo-Souza, Patricia Savio ; Scapulatempo-Neto, Cristovam ; Herbster, Suellen ; Boccardo, Enrique ; Termini, Lara
Total Authors: 8
Document type: Journal article
Source: ACTA CYTOLOGICA; v. 65, n. 2, p. 10-pg., 2020-12-03.
Abstract

Introduction: The main risk factor for the development of cervical cancer (CC) is persistent infection by human papillomavirus (HPV) oncogenic types. In order to persist, HPV exhibits a plethora of immune evasion mechanisms. PI3/Elafin (Peptidase Inhibitor 3) is an endogenous serine protease inhibitor involved in epithelial protection against pathogens. PI3/Elafin's role in CC is still poorly understood. Materials and Methods: In the present study, we addressed PI3/Elafin protein detection in 123 CC samples by immunohistochemistry and mRNA expression in several datasets available at Gene Expression Omnibus and The Cancer Genome Atlas platforms. Results: We observed that PI3/Elafin is consistently downregulated in CC samples when compared to normal tissue. Most of PI3/Elafin-positive samples exhibited this protein at the plasma membrane. Besides, high PI3/Elafin expression at the cellular membrane was more frequent in in situ stages I + II than in invasive cervical tumor stages III + IV. This indicates that PI3/Elafin expression is gradually lost during the CC progression. Of note, advanced stages of CC were more frequently associated with a more intense PI3/Elafin reaction in the nuclei and cytoplasm. Conclusion: Our results suggest that PI3/Elafin levels and subcellular localization may be used as a biomarker for CC severity. (AU)

FAPESP's process: 08/57889-1 - Institute of Science and Technology to study Diseases Associated with Papillomavirus
Grantee:Luisa Lina Villa
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 18/22097-0 - Malignant neoplasms of the 18 cities of Barretos Regional Health District (RhD), Sao Paulo, Brazil: the importance of a population-based cancer registry
Grantee:Allini Mafra da Costa
Support Opportunities: Scholarships in Brazil - Post-Doctoral