| Full text | |
| Author(s): |
de Andrade Carvalho, Vinicius Miessler
;
Nakahara, Thiago Seike
;
Papes, Fabio
Total Authors: 3
|
| Document type: | Journal article |
| Source: | JOVE-JOURNAL OF VISUALIZED EXPERIMENTS; v. N/A, n. 170, p. 16-pg., 2021-04-01. |
| Abstract | |
Animals rely on chemical communication to convey and perceive relevant environmental information, ranging from assessment of food quality to detection of available mating partners or threats. In mice, this task is executed primarily by the olfactory system and its underlying subsystems, including the main and accessory olfactory systems. Both have peripheral organs populated by sensory neurons expressing G-protein coupled receptors able to bind chemical cues that reach the nasal cavity. Even though the molecular characteristics of these receptors is well understood, little is known about their cognate specific ligands. The method described here combines in situ hybridization detection of olfactory or vomeronasal receptors with immunodetection of phosphorylated ribosomal protein S6 (pS6) - a marker of neuronal activation. This protocol was devised to identify neurons activated after a single event of exposure to purified or complex chemical stimuli detected by the olfactory organs. Importantly, this technique allows the investigation of neurons triggered in biologically relevant contexts. Ideally, this method should be used to probe the molecular biology of the olfactory system and to study olfactory behaviors. (AU) | |
| FAPESP's process: | 12/01689-0 - Molecular study of the epigenome involved in the regulation of olfactory receptor gene expression |
| Grantee: | Vinicius Miessler de Andrade Carvalho |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |
| FAPESP's process: | 15/50371-0 - Mechanisms underkying how social experience alters hormone levels, sensory signaling and behavior |
| Grantee: | Fabio Papes |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 09/00473-0 - Molecular biology of the olfactory system in mammals: study on the detection of odors and their neural representation in the brain |
| Grantee: | Fabio Papes |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 14/25594-3 - Optogenetic manipulation of olfactory circuits |
| Grantee: | Vinicius Miessler de Andrade Carvalho |
| Support Opportunities: | Scholarships abroad - Research Internship - Doctorate (Direct) |
| FAPESP's process: | 12/21786-0 - Ascertainment and analysis of olfactory maps in the brain |
| Grantee: | Vinicius Miessler de Andrade Carvalho |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |
| FAPESP's process: | 12/04026-1 - Functional and Molecular Characterization of OR class receptors expressed in the mammalian Vomeronasal Organ. |
| Grantee: | Thiago Seike Picoreti Nakahara |
| Support Opportunities: | Scholarships in Brazil - Master |