Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Assessing reservoir host status in leishmaniasis with special reference to the infectiousness of Leishmania (Viannia) braziliensis infections in wild rodents

Full text
Author(s):
Jeffrey J. Shaw [1] ; José F. Marinho-Júnior [2] ; Orin Courtenay [3] ; Sinval P. Brandão-Filho [4]
Total Authors: 4
Affiliation:
[1] Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Parasitologia - Brasil
[2] Fundação Oswaldo Cruz. Instituto Aggeu Magalhães - Brasil
[3] University of Warwick. School of Life Sciences and the Zeeman Institute - Reino Unido
[4] Fundação Oswaldo Cruz. Instituto Aggeu Magalhães - Brasil
Total Affiliations: 4
Document type: Journal article
Source: Revista da Sociedade Brasileira de Medicina Tropical; v. 56, 2023-12-15.
Abstract

ABSTRACT Molecular methods have been responsible for a notable increase in the detection of Leishmaniinae infections in wild animals. Determining their infectiousness is of paramount importance in evaluating their epidemiological significance. One of the most efficient ways of determining infectiousness for vector borne diseases is xenodiagnosis with the appropriate vector. However, this is logistically very difficult to accomplish in the field, and an ideal solution is to find a molecular surrogate for xenodiagnosis. In this review we discuss different approaches to the problem by focusing on the infectiousness of Leishmania (Viannia) braziliensis in rodents under laboratory and field conditions. Comparisons with similar studies for other Leishmania species emphasizes that there are pivotal differences in the infectiousness and the importance of asymptomatic infections in different hosts. Potentially the most promising surrogate is the real time quantitative PCR (qPCR). However, its success depends on choosing a tissue that relates to the vector’s feeding location and the parasite’s tissue tropism. This requires detailed knowledge of the infection of each species in its wild hosts. We conclude that for L. (V.) braziliensis infections in wild rodents the tissue of choice for a molecular xenodiagnostic test, based on the qPCR is blood, providing that a significant number of samples must be examined. (AU)

FAPESP's process: 22/08550-9 - International Congress on Leishmania & Leishmaniasis- WORLDLEISH7
Grantee:Jeffrey Jon Shaw
Support Opportunities: Research Grants - Meeting - Abroad