Antifungal compound produced by the cassava endoph... - BV FAPESP
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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Antifungal compound produced by the cassava endophyte Bacillus pumilus MAIIIM4a

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Author(s):
Flávia Mandolesi Pereira de Melo ; Marli Fátima Fiore [2] ; Luiz Alberto Beraldo de Moraes [3] ; Maria Estela Silva-Stenico [4] ; Shirlei Scramin [5] ; Manoel de Araújo Teixeira ; Itamar Soares de Melo [7]
Total Authors: 7
Document type: Journal article
Source: Scientia Agricola; v. 66, n. 5, p. 583-592, 2009-10-00.
Abstract

In the search for new organisms and new secondary metabolites, a study was conducted to evaluate the diversity of endophytic bacteria from ethnovarieties of cassava cultivated by Brazilian Amazon Indian tribes and also to study the secondary metabolites produced by a Bacillus pumilus strain. Sixty seven cassava endophytic bacteria were subjected to 16S rRNA sequencing and FAME analysis. The bacterial profile revealed that 25% of all endophytic isolates belonged to the genus Bacillus. The isolate B. pumilus MAIIIM4a showed a strong inhibitory activity against the fungi Rhizoctonia solani, Pythium aphanidermatum and Sclerotium rolfsii. Secondary metabolites of this strain were extracted using hexane, dichloromethane and ethyl acetate. Extracts were subjected to bioautography and LC/MS analysis, which allowed the identification of pumilacidin, an antifungal compound produced by B. pumilus MAIIIM4a. The bacterial endophytic localization was confirmed by cassava cell tissue examination using scanning electron microscopy. (AU)

FAPESP's process: 99/09177-1 - Biodiversity of endophytic microorganisms and their biotechnological potential
Grantee:Itamar Soares de Melo
Support Opportunities: BIOTA-FAPESP Program - Thematic Grants