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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

FVB/NJ strain as a mouse model for cutaneous leishmaniasis by Leishmania (L.) amazonensis

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Author(s):
Guilherme Moreira Paiva Carrara [1] ; Beatriz Simonsen Stolf [2]
Total Authors: 2
Affiliation:
[1] Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Parasitologia - Brasil
[2] Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Parasitologia - Brasil
Total Affiliations: 2
Document type: Journal article
Source: Memórias do Instituto Oswaldo Cruz; v. 119, 2024-03-15.
Abstract

BACKGROUND Leishmaniases encompass a spectrum of neglected diseases caused by parasites of the genus Leishmania, grouped in two forms: tegumentary and visceral leishmaniasis. OBJECTIVES In this study, we propose Friend Virus B NIH Jackson (FVB/NJ) mouse strain as a new experimental model of infection with Leishmania (Leishmania) amazonensis, the second most prevalent agent of tegumentary leishmaniasis in Brazil. METHODS AND FINDINGS We performed in vitro infections of FVB/NJ macrophages and compared them with BALB/c macrophages, showing that BALB/c cells have higher infection percentages and a higher number of amastigotes/cell. Phagocytosis assays indicated that BALB/c and FVB/NJ macrophages have similar capacity to uptake parasites after 5 min incubations. We also investigated promastigotes’ resistance to sera from FVB/NJ and BALB/c and observed no difference between the two sera, even though FVB/NJ has a deficiency in complement components. Finally, we subcutaneously infected FVB/NJ and BALB/c mice with 2 × 106 parasites expressing luciferase. Analysis of lesion development for 12 weeks showed that FVB/NJ and BALB/c mice have similar lesion profiles and parasite burdens. MAIN CONCLUSIONS This work characterises for the first time the FVB/NJ mouse as a new model for tegumentary leishmaniasis caused by Leishmania (L.) amazonensis. (AU)

FAPESP's process: 21/08915-4 - Identification of factors related to phagocytosis and survival of L. amazonensis by comparison of proteomes and analysis of mutants by CRISPR-Cas9
Grantee:Beatriz Simonsen Stolf
Support Opportunities: Regular Research Grants
FAPESP's process: 18/14972-8 - Role of sCD100 in in vitro and in vivo infection by different species of Leishmania
Grantee:Beatriz Simonsen Stolf
Support Opportunities: Regular Research Grants