| Full text | |
| Author(s): |
Karina de Almeida Lins
[1]
;
Cristiane Santos Cruz Piveta
[2]
;
Carlos Emilio Levy
[3]
;
Marina Rovani Drummond
[4]
;
Luciene Silva dos Santos
[5]
;
Alessandra Sussulini
[6]
;
Paulo Eduardo Neves Ferreira Velho
[7]
Total Authors: 7
|
| Affiliation: | [1] Universidade Estadual de Campinas. Faculdade de Ciências Médicas. Departamento de Dermatologia - Brasil
[2] Universidade Estadual de Campinas. Faculdade de Ciências Médicas. Departamento de Patologia Clínica - Brasil
[3] Universidade Estadual de Campinas. Faculdade de Ciências Médicas. Departamento de Patologia Clínica - Brasil
[4] Universidade Estadual de Campinas. Faculdade de Ciências Médicas. Departamento de Dermatologia - Brasil
[5] Universidade Estadual de Campinas. Faculdade de Ciências Médicas. Departamento de Dermatologia - Brasil
[6] Universidade Estadual de Campinas. Instituto de Química. Departamento de Química Analítica - Brasil
[7] Universidade Estadual de Campinas. Faculdade de Ciências Médicas. Departamento de Dermatologia - Brasil
Total Affiliations: 7
|
| Document type: | Journal article |
| Source: | Revista do Instituto de Medicina Tropical de São Paulo; v. 66, 2024-02-05. |
| Abstract | |
ABSTRACT Bartonella spp. are bacteria responsible for neglected diseases worldwide. Bartonella henselae is the species most associated with human infections. It is associated with a large spectrum of clinical manifestations and is potentially fatal. The identification of Bartonella spp. is considered a challenge in clinical routine. These bacteria are fastidious, and the time required to isolate them varies from one to six weeks. MALDI-TOF mass spectrometry has emerged as an application for research on Bartonella spp. , and has still been little explored. We investigated whether three different B. henselae strains with different growth times—14 and 28 days—could be correctly identified by MALDI-TOF mass spectra fingerprint comparison and matching. We found that the spectra from strains with different growth times do not match each other, leading to misidentification. We suggest creating database entries with multiple spectra from strains with different growth times to increase the chances of accurate identification of Bartonella spp. by MALD-TOF MS. (AU) | |
| FAPESP's process: | 18/12565-6 - Vectors of Chagas' disease as potential transmitters of Bartonella spp. Abstract |
| Grantee: | Marina Rovani Drummond |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |