Development and Validation of a Method to Analyze Fentanyl and Its Analogues in Postmortem Blood Samples by LC-MS/MS

Author(s)

Taís B. Rodrigues ^[1] ; Márcio H. Matsubara ^[2] ; Elvis M. Aquino ^[3] ; Victor A. P. Gianvecchio ^[4] ; Damila R. Morais ^[5] ; Jose Luiz Costa

Affiliation

^[1] Universidade Estadual de Campinas (UNICAMP). Faculdade de Ciências Médicas. Centro de Informação e Assistência Toxicológica de Campinas (CIATox-Campinas) - Brasil

^[2] Superintendência da Polícia Técnico Científica do Estado de São Paulo. Instituto Médico Legal. Núcleo de Toxicologia Forense - Brasil

^[3] Superintendência da Polícia Técnico Científica do Estado de São Paulo. Instituto Médico Legal. Núcleo de Toxicologia Forense - Brasil

^[4] Superintendência da Polícia Técnico Científica do Estado de São Paulo. Instituto Médico Legal. Núcleo de Toxicologia Forense - Brasil

^[5] Shimadzu do Brasil - Brasil

• Document type: Journal article
• Source: Journal of the Brazilian Chemical Society; v. 35, n. 7 2024-02-12.

Abstract

The increase in the number of users, hospitalizations and deaths due to overdose of synthetic opioids made the scientific community to treat this problem as “the opioid crisis”, justifying the need for the development of analytical strategies to identify and quantify these drugs. Even with the global discussion, there is no Brazilian data regarding opioids consumption, addiction or fatal intoxication cases. The aim of this work was to develop, validate and applied an analytical method based on liquid chromatography-tandem mass spectrometry for determination of fentanyl and seven analogues (acetyl norfentanyl, acetyl fentanyl, thiofentanyl, acrylfentanyl, furanylfentanyl, carfentanil and valerylfentanyl) in postmortem blood samples, to the routine analysis of the Forensic Toxicology Laboratory of São Paulo State Police. Linearity was evaluated from 1-500 ng mL-1 (correlation coefficient (r) value (r ≥ 0.99), 1/x weight linear regression), with limit of quantification of 1 ng mL-1. The method imprecision, bias and matrix effect were lower than 19.7%. No interference or carryover were observed, and extraction yield was greater than 57.7%. Analysis of postmortem blood samples (n = 1,359) showed that fentanyl was the most frequently detected opioid. The developed method proved to be useful for routine analysis of the Forensic Toxicology Laboratory. (AU)

• FAPESP's process: 18/00432-1 - The Toxicology of New Psychoactive Substances (NSP): epidemiology of consumption by the analysis of hair and oral fluid samples
• Grantee: José Luiz da Costa
• Support Opportunities: Regular Research Grants
• FAPESP's process: 20/10809-5 - Microextraction techniques in forensic toxicology
• Grantee: José Luiz da Costa
• Support Opportunities: Regular Research Grants