Recombinant cellobiose dehydrogenase from Thermothelomyces thermophilus : Its functional characterization and applicability in cellobionic acid production

Author(s)

Oliva, Bianca ; Velasco, Josman ; Berto, Gabriela Leila ; Polikarpov, Igor ; de Oliveira, Leandro Cristante ; Segato, Fernando

• Document type: Journal article
• Source: Bioresource Technology; v. 402, p. 11-pg., 2024-05-03.

Abstract

The fungus Thermothelomyces thermophilus is a thermotolerant microorganism that has been explored as a reservoir for enzymes (hydrolytic enzymes and oxidoreductases). The functional analysis of a recombinant cellobiose dehydrogenase (MtCDHB) from T. thermophilus demonstrated a thermophilic behavior, an optimal pH in alkaline conditions for inter-domain electron transfer, and catalytic activity on cellooligosaccharides with different degree of polymerization. Its applicability was evaluated to the sustainable production of cellobionic acid (CBA), a potential pharmaceutical and cosmetic ingredient rarely commercialized. Dissolving pulp was used as a disaccharide source for MtCDHB. Initially, recombinant exoglucanases (MtCBHI and MtCBHII) from T. thermophilus hydrolyzed the dissolving pulp, resulting in 87% cellobiose yield, which was subsequently converted into CBA by MtCDHB, achieving a 66% CBA yield after 24 h. These findings highlight the potential of MtCDHB as a novel approach to obtaining CBA through the bioconversion of a plant-based source. (AU)

• FAPESP's process: 14/18714-2 - Enzymatic oxidation of sugarcane bagasse: discovery, characterization and new application of oxidative enzymes active in carbohydrates, applied to the enhancement of a fungal cell factory
• Grantee: Fernando Segato
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Young Investigators Grants
• FAPESP's process: 19/06663-8 - Biochemical and functional studies of cellobiohydrolases from basidiomycetes of white-rot fungi with biotechnological potential
• Grantee: Bianca Oliva
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 19/01165-0 - New recombinant PMOS from Aspergillus fumigatus: biochemical characterization and its effect on saccharification of sugarcane bagasse with different pre-treatments
• Grantee: Josman Andrey Velasco Mendoza
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 22/04227-9 - Multiuser equipment granted in the proposal 2021/06679-1: Dionex ICS600 Capillary HPIC system
• Grantee: Fernando Segato
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 21/06679-1 - Enzymatic oxidation of sugarcane bagasse 2: exploring the interaction between LPMOs and its redox partner CDHs aiming for the development of more efficient enzymes for insertion into an engineered cell factory
• Grantee: Fernando Segato
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Young Investigators Grants - Phase 2
• FAPESP's process: 19/22284-7 - Biochemical, functional characterization and application of polysaccharide lytic monoxygenases identified in transcriptome and secretoma studies of filamentous fungi cultivated in sugarcane bagasse
• Grantee: Fernando Segato
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants