| Full text | |
| Author(s): Show less - |
Munhoz, Lauriene Luiza de Souza
;
Guillens, Luiz Carlos
;
Alves, Beatriz Candido
;
do Nascimento, Maria Gabriela Oliveira Ferreira
;
Meneguin, Andreia Bagliotti
;
Carbinatto, Fernanda Mansano
;
Arruda, Gabriela
;
Barud, Hernane da Silva
;
de Aro, Andrea
;
Casagrande, Laura de Roch
;
Silveira, Paulo Cesar Lock
;
Andrade, Thiago Antonio Moretti
;
dos Santos, Glaucia Maria Tech
;
Caetano, Guilherme Ferreira
Total Authors: 14
|
| Document type: | Journal article |
| Source: | Acta Cirurgica Brasileira; v. 39, p. 12-pg., 2024-01-01. |
| Abstract | |
Purpose: Bacterial cellulose (BC) has shown high capacity for the treatment of wounds and burns, providing a moisty environment. Calcium alginate can be associated with BC to create gels that aid in wound debridement and contribute to appropriate wound healing. This study is aimed at characterizing and evaluating the use of bacterial cellulose/alginate gel in skin burns in rats. Methods: Cellulose and cellulose/alginate gels were compared regarding the capacity of liquid absorption, moisture, viscosity, and potential cytotoxicity. The 2nd degree burns were produced using an aluminum metal plate (2.0cm) at 120 degrees C for 20s on the back of rats. The animals were divided into non-treated, CMC(Carboxymethylcellulose), Cellulose(CMC with bacterial cellulose), and Cellulose/alginate(CMC with bacterial cellulose and alginate). The animals received topical treatment 3 times/week. Biochemical (MPO, NAG and oxidative stress), histomorphometry and immunohistochemical assays (IL-1 beta IL-10 and VEGF) were conducted on the 14th, 21st, 28th, and 35th days. Results: Cellulose/Alginate gel showed higher absorption capacity and viscosity compared to Cellulose gel, with no cytotoxic effects. Cellulose/alginate presented lower MPO values, a higher percentage of IL-10, with greater and balanced oxidative stress profile. Conclusion: The use of cellulose/alginate gel reduced neutrophils and macrophage activation and showed greater anti-inflammatory response, which can contribute to healing chronic wounds and burns. (AU) | |
| FAPESP's process: | 21/11965-3 - Center for the sustainable management of pests, diseases and weeds |
| Grantee: | Mário Eidi Sato |
| Support Opportunities: | Research Grants - Science Centers for Development |