Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Abdominal hyperalgesia in secretory phospholipase A(2)-induced rat pancreatitis: Distinct roles of NK1 receptors

Full text
Author(s):
Camargo, Enilton A. [1, 2] ; Zanoni, Cristiane I. [1] ; Toyama, Marcos H. [3] ; Muscara, Marcelo N. [1] ; Docherty, Reginald J. [4] ; Costa, Soraia K. P. [1]
Total Authors: 6
Affiliation:
[1] Univ Fed Sergipe, Dept Physiol, BR-49100000 Aracaju, SE - Brazil
[2] Univ Sao Paulo, Inst Biosci ICB I, Dept Pharmacol, BR-05508900 Sao Paulo - Brazil
[3] Univ Sao Paulo State UNESP, Sao Vicente Unit, Sao Paulo - Brazil
[4] Kings Coll London, Sch Biomed & Hlth Sci, Wolfson Ctr Age Related Dis, London SE1 9RT - England
Total Affiliations: 4
Document type: Journal article
Source: EUROPEAN JOURNAL OF PAIN; v. 15, n. 9, p. 900-906, OCT 2011.
Web of Science Citations: 8
Abstract

We investigated the potential of secretory phospholipase A(2) (sPLA(2))-induced pancreatitis to promote abdominal hyperalgesia, as well as to depolarize sensory fibres in vitro using a grease-gap technique. Pancreatitis was induced by the injection of sPLA(2) from Crotalus durissus terrificus (sPLA(2) Cdt, 300 mu g kg(-1)) venom into the common bile duct of rats. Pancreatic inflammatory signs, serum amylase levels and abdominal hyperalgesia were evaluated in rats treated or not with SR140333, a tachykinin NK(1) receptor antagonist. Injection of sPLA(2) Cdt caused pancreatic oedema formation and increased pancreatic neutrophil infiltration and serum amylase at 4 h, which returned to normality by 24 h, except for the neutrophil infiltration, which was still increased at this time point. Animals injected with sPLA(2) exhibited a lower withdrawal threshold to electronic von Frey stimulation in the upper abdominal region at 4 h, but not 24 h, post-injection when compared with saline-injected rats. Pre-treatment of animals with SR140333 significantly reduced the sPLA(2) Cdt-induced abdominal hyperalgesia, without affecting the other parameters. Neither sPLA(2) Cdt nor sPLA(2) from Naja mocambique mocambique venom depolarized capsaicin-sensitive sensory fibres from rat vagus nerve, but they decreased the propagated compound action potentials in both A and C fibres. These data show for the first time that NK(1) receptors play an important role in the early abdominal hyperalgesia in a rat model of sPLA(2)-induced pancreatitis, suggesting that these receptors are of importance in the development of pain in the pancreatitis condition. We also provide evidence that sPLA(2)s do not directly depolarize sensory fibres in vitro. (C) 2011 European Federation of International Association for the Study of Pain Chapters. Published by Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 07/00529-0 - Pharmacological investigation of neurovascular mechanisms involved in the secretory phospholipases A2-induced acute pancreatitis
Grantee:Soraia Katia Pereira Costa
Support Opportunities: Regular Research Grants