| Full text | |
| Author(s): Show less - |
Cabral, Rosa M.
[1]
;
Branco, Erika
[2]
;
Rizzo, Marcia Dos S.
[1]
;
Ferreira, Guilherme J.
[3]
;
Gregores, Guilherme B.
[4]
;
Samoto, Vivian Y.
[4]
;
Stopiglia, Angelo J.
[4]
;
Maiorka, Paulo C.
[5]
;
Fioretto, Emerson T.
[6]
;
Capelozzi, Vera L.
[7]
;
Borges, Joao B.
[8]
;
Gomes, Susimeire
[8]
;
Beraldo, Marcelo A.
[8]
;
Carvalho, Carlos R. R.
[8]
;
Miglino, Maria A.
[4]
Total Authors: 15
|
| Affiliation: | [1] Fed Univ Piaui UFPI, Dept Vet Clin & Surg, Teresina, PI - Brazil
[2] Fed Rural Univ Amazonia UFRA, Inst Anim Hlth & Prod ISPA Fac Vet Med, Belem, Para - Brazil
[3] Univ Fed Piaui, Dept Morphol, Bom Jesus, PI - Brazil
[4] Univ Sao Paulo, Fac Vet Med & Anim Sci FMVZ USP, Dept Surg, Sao Paulo - Brazil
[5] Univ Sao Paulo, Fac Vet Med & Anim Sci FMVZ USP, Dept Pathol, Sao Paulo - Brazil
[6] Fed Univ Sergipe UFS, Ctr Biol & Hlth Sci, Lab Cellular & Struct Biol, Dept Morphol, Aracaju, SE - Brazil
[7] Univ Sao Paulo, Fac Med FM USP, Lab Histopathol & Immunol, Sao Paulo - Brazil
[8] Univ Sao Paulo, Fac Med FM USP, Lab Pneumol LIM 9, Sao Paulo - Brazil
Total Affiliations: 8
|
| Document type: | Journal article |
| Source: | MICROSCOPY RESEARCH AND TECHNIQUE; v. 74, n. 10, p. 957-962, OCT 2011. |
| Web of Science Citations: | 9 |
| Abstract | |
Parte superior do formulario Digite um texto ou endereco de um site ou traduza um documento. The aim of this study is to evaluate the histological changes in lung parenchyma of pigs affected by interstitial lung disease induced after the infusion of bone marrow mononuclear cells (BMMCs). Ten female swines were submitted to pulmonary fibrosis induced by a single dose of intratracheal bleomicine sulfate. Animals were arranged into two groups: Group 1: induced-disease control and Group 2: cell therapy using BMMCs. Both groups were clinically evaluated for 180 days. High-resolution computed tomography (HRCT) was performed at 90 and 180 days. BMMC sampling was performed in cell therapy group at 90 days. Euthanasia was performed, and samples were collected for histology and immunohistochemistry. The 90-days HRCT demonstrated typical interstitial lesions in pulmonary parenchyma similarly to human disease. The 180-days HRCT in Group 1 demonstrated advanced stages of the disease when compared with Group 2. Immunohistochemistry analysis suggests the presence of pre-existent vessels and neoformed vessels as well as predominant young cells in the injured parenchyma of Group 2. Immunohistochemistry analysis suggests that cell therapy would promote a reconstructive response. Histology and HRCT analysis suggest a positive application of swine as a model for a bleomicine inducing of fibrotic interstitial pulmonary disease. Microsc. Res. Tech. 74:957-962, 2011. (C) 2011 Wiley-Liss, Inc. (AU) | |