Maternal preconception glucose intolerance and fatty acid intake from conception to weaning: impact on offspring energy homeostasis in both male and female

Author(s)

de Souza, Esther Alves ; Mennitti, Lais Vales ; Santamarina, Aline Boveto ; Minari, Tatiana Palotta ; Juca, Andrea ; Sertorio, Marcela Nascimento ; Pisani, Luciana Pellegrini

• Document type: Journal article
• Source: EUROPEAN JOURNAL OF NUTRITION; v. 63, n. 8, p. 12-pg., 2024-09-04.

Abstract

Environmental factors in the early life stages can lead the descendant to adaptations in gene expression, permanently impacting several structures and organs. The amount and quality of fatty acids in the maternal diet in pregnancy and lactation were found to impact offspring metabolism. So, maternal diet and insulin resistance can affect the male and female descendants through distinct pathways and at different time points. We hypothesized that maternal high-fat diet (HFD) intake before conception and an adequate amount of different fatty acids intake during pregnancy and lactation could influence the energy homeostasis system of 21-day-old offspring. Female rats received control diet (C) or HFD (HF) for 8 weeks before pregnancy. During pregnancy and lactation C group remained with same diet (C-C), HF group were distributed into 4 groups and received C diet (HF-C), normolipidic diet based on saturated fatty acids (HF-S) or based on polyunsaturated fatty acids n-3 (HF-P) or remained in same diet (HF-HF). Maternal HFD in preconception, pregnancy, and lactation (HF-HF) led to lower glucagon-like peptide-1 levels in male (HF-HF21) compared to other groups (C-C21, HF-C21, and HF-P21) and compared to HF-HF21 females. Neuropeptide YY levels were higher in the HF-HF21, HF-C21, and HF-S21 male offspring compared to HF-P21. HF-P21 was similar to C-C21. Positive correlations were found among the energy homeostasis markers genes expressed in the offspring hypothalamus. Maternal diet changes to adequate quantities of fatty acids during pregnancy and lactation showed less impaired results but was not entirely avoided. A maternal diet based on PUFA n-3 during pregnancy and lactation seems to reverse the damage of an HFD in preconception. These results of homeostasis energy system disturbance in the offspring at weaning give us clues about changes that precede the onset of the disease in adult life - adding notes to the knowledge for future investigations of prevention and treatment of chronic diseases. (AU)

• FAPESP's process: 19/09359-8 - EPIGENETIC PROFILE AND MITOCHONDRIAL PHENOTYPE OF THE 21-DAY-OLD OFFSPRING FROM OBESE DAMS FED WITH DIFFERENT TYPES OF FATTY ACIDS DURING PREGNANCY AND LACTATION
• Grantee: Laís Vales Mennitti
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 22/05529-9 - Effect of maternal hyperlipidic diet on male and female offspring health focussed on epigenetic biomarkers and energetic homeostasis
• Grantee: Luciana Pellegrini Pisani
• Support Opportunities: Regular Research Grants
• FAPESP's process: 21/10375-8 - Influence of intake of different types of fatty acids during pregnancy and lactation in obese rats on epigenetics and energy homeostasis regulation of offspring after lactation
• Grantee: Esther Alves de Souza
• Support Opportunities: Scholarships in Brazil - Doctorate