| Full text | |
| Author(s): |
Delgado-Garcia, Lina M.
;
Ojalvo-Sanz, Ana C.
;
Nakamura, Thabatta K. E.
;
Martin-Lopez, Eduardo
;
Porcionatto, Marimelia
;
Lopez-Mascaraque, Laura
Total Authors: 6
|
| Document type: | Journal article |
| Source: | Biological Research; v. 57, n. 1, p. 16-pg., 2024-08-14. |
| Abstract | |
Brain damage triggers diverse cellular and molecular events, with astrocytes playing a crucial role in activating local neuroprotective and reparative signaling within damaged neuronal circuits. Here, we investigated reactive astrocytes using a multidimensional approach to categorize their responses into different subtypes based on morphology. This approach utilized the StarTrack lineage tracer, single-cell imaging reconstruction and multivariate data analysis. Our findings identified three profiles of reactive astrocyte responses, categorized by their effects on cell size- and shape- related morphological parameters: "moderate", "strong," and "very strong". We also examined the heterogeneity of astrocyte reactivity, focusing on spatial and clonal distribution. Our research revealed a notable enrichment of protoplasmic and fibrous astrocytes within the "strong" and "very strong" response subtypes. Overall, our study contributes to a better understanding of astrocyte heterogeneity in response to an injury. By characterizing the diverse reactive responses among astrocyte subpopulations, we provide insights that could guide future research aimed at identifying novel therapeutic targets to mitigate brain damage and promote neural repair. (AU) | |
| FAPESP's process: | 19/09183-7 - Characterization of astroglial clonal responses in experimental animal models of brain injury |
| Grantee: | Lina Maria Delgado Garcia |
| Support Opportunities: | Scholarships abroad - Research Internship - Doctorate |
| FAPESP's process: | 16/19084-8 - Crosstalk among Wnt, SHH and Notch and the efect of Gal3 protein on the regulation of the proliferation of derived-neural stem cells reactive astrocytes |
| Grantee: | Lina Maria Delgado Garcia |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 18/12605-8 - Development of brain-on-a-chip microplataforms for in vitro modeling of the central nervous system |
| Grantee: | Marimélia Aparecida Porcionatto |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 22/04258-1 - Exploring neurovascular unit adaptations in the regulation of apoptosis and glial response after brain injury |
| Grantee: | Lina Maria Delgado Garcia |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |