Development of brain-on-a-chip microplataforms for in vitro modeling of the central nervous system

• Grant number: 18/12605-8
• Support type: Research Projects - Thematic Grants
• Duration: November 01, 2019 - October 31, 2024
• Field of knowledge: Biological Sciences - Morphology - Cytology and Cell Biology
• Principal researcher: Marimélia Aparecida Porcionatto
• Grantee: Marimélia Aparecida Porcionatto
• Home Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Pesquisadores principais: Lucimara Gaziola de la Torre ; Paulo Schor
• Assoc. researchers: Alexander Birbrair ; Alexander Henning Ulrich ; Ana Luíza Garcia Millás Massaguer ; Angelo Luiz Gobbi ; Beatriz de Oliveira Monteiro ; Giselle Zenker Justo ; Isaias Glezer ; Jean Faber Ferreira de Abreu ; Kil Sun Lee ; Laura Lopez Mascaraque ; Laura Nicoleti Zamproni ; Lucimara Gaziola de la Torre ; Luiz Eugenio Araujo de Moraes Mello ; Paulo Schor ; Renato Sousa Lima ; Sang Won Han ; Su Ryon Shin
• Associated grant(s): 20/05289-2 - In vitro and in silico modeling of COVID-19, AP.R
• Associated scholarship(s): 21/14327-8 - Subarachnoid haemorrhage-in-a-chip, BP.PD; 21/07024-9 - Evaluation of the use of neuroblastoma cell line Neuro2a as a study model for inhibition of axonal growth in 2D and 3D, BP.IC; 21/10787-4 - Study of the synergy between PROK2 and CXCL12 in the chemotaxis of neuroblasts and endothelial cells in bioprinted tissue, BP.IC; + associated scholarships 20/12454-0 - 3D bioprinting of reactive astrocyte-derived neural stem cells, BP.DR; 21/02795-7 - Brain-on-a-chip microfluidic platform for mimicking the blood-brain barrier, BP.DD; 20/07402-0 - 3D bioprinted model of the blood-brain-barries to study the development of Alzheimer´s s disease, BP.IC - associated scholarships

Abstract

The concept of organ-on-a-chip refers to different types of in vitro modeling or reproduction of tissues and organs by combining cells and biocompatible material. This approach has become an attractive alternative for various studies, such as drug testing. The initial push was the worldwide effort to reduce the number of lab animals used in research, by the application of the 3R's - replacement, reduction, and refinement, although organs-on-a-chip have proven to have broader applications, such as the study organ development and drug testing. Microplataforms to model the central nervous system (CNS), known as brain-on-a-chip, have also been developed, as a combination of neural stem cells or induced pluripotency stem cells (iPSCs) cultured on biocompatible materials with the introduction of microfluidic chambers to mimic blood flow and the blood-brain-barrier, and the cerebrospinal fluid. Brain-on-a-chip microplataforms used for in vitro studies of the CNS allow several different applications such as drug testing and the study of cellular and molecular mechanisms of neurodegenerative diseases. In this scenario, our goal is to develop microplataforms to study CNS normal development, cellular and molecular mechanisms of disorders of the CNS, and neuronal connectivity, using 3D bioprinting, microfluidic chambers and microelectrode arrays (MEAs). (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RIBEIRO, TAIS NOVAKI; DELGADO-GARCIA, LINA MARIA; PORCIONATTO, MARIMELIA A.. Notch1 and Galectin-3 Modulate Cortical Reactive Astrocyte Response After Brain Injury. FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY, v. 9, JUN 16 2021. (16/25737-4, 18/05846-9, 16/19084-8, 18/12605-8, 15/19231-8)

DE MELO, BRUNA A. G.; JODAT, YASAMIN A.; CRUZ, ELISA M.; BENINCASA, JULIA C.; SHIN, SU RYON; PORCIONATTO, MARIMELIA A.. Strategies to use fibrinogen as bioink for 3D bioprinting fibrin-based soft and hard tissues. Acta Biomaterialia, v. 117, p. 60-76, NOV 2020. (18/23039-3, 18/12605-8)

DE MELO, BRUNA A. G.; CRUZ, ELISA M.; RIBEIRO, TAIS N.; MUNDIM, V, MAYARA; PORCIONATTO, MARIMELIA A.. 3D Bioprinting of Murine Cortical Astrocytes for Engineering Neural-Like Tissue. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, n. 173, JUL 2021. (18/23039-3, 18/12605-8)

ZAMPRONI, LAURA N.; MUNDIM, MAYARA T. V. V.; PORCIONATTO, MARIMELIA A.. eurorepair and Regeneration of the Brain: A Decade of Bioscaffolds and Engineered Microtissu. FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY, v. 09, APR 7 2021. (18/12605-8, 13/16533-8, 14/23797-4, 12/06810-1, 14/00927-0)