Scholarship 23/14105-0 - Bioimpressão, Fatores quimiotáticos - BV FAPESP
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Identification of chemotactic factors responsible for the migratory control of hiPSCs-NPC cells in a 3D bioprinted model

Grant number: 23/14105-0
Support Opportunities:Scholarships in Brazil - Doctorate
Start date until: March 01, 2024
End date until: January 31, 2027
Field of knowledge:Biological Sciences - Biochemistry - Molecular Biology
Principal Investigator:Marimélia Aparecida Porcionatto
Grantee:Natália Heloísa de Oliveira
Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
Associated research grant:18/12605-8 - Development of brain-on-a-chip microplataforms for in vitro modeling of the central nervous system, AP.TEM

Abstract

The difficult access to human tissue and the considerable differences observed between the human cortex and other mammal cortexes highlight the need to produce in vitro models that recapitulate the initial stages of human cortical development. Through the distribution of cells and biomaterials, 3D bioprinting allowsthe construction of microenvironments that support cell-cell and cell-matrix interactions, promoting the differentiation and migration of neuroprogenitors. In this context, this project's main objective is to identify the chemotactic factors responsible for the migratory control of neuro progenitors derived from human pluripotent cells (hiPSC-NPCs) and their signaling pathways in the 3D bioprinted model. The expression of genes coding for chemotactic factors important for cortical migration will be ascertained through gene expression analysis by RT-PCR. The ELISA assay will verify the presence of secreted chemotactic factors in the culture medium. The expression of specific receptors for the chemotactic factors will be observed through immunofluorescence analysis. The role of chemotactic factors CXCL12, Sema3A, and PROK2 in NPC migration, will be ascertained through time-lapse microscopy analysis after pharmacological inhibition of receptors and signaling pathways.

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