| Full text | |
| Author(s): |
Lessa, Carlos J. A.
;
Ruiz, Gilia C. M.
;
Alessio, Priscila
;
Constantino, Carlos J. L.
Total Authors: 4
|
| Document type: | Journal article |
| Source: | ACS APPLIED NANO MATERIALS; v. 7, n. 11, p. 11-pg., 2024-05-21. |
| Abstract | |
This study explored the effects of bisphenol A (BPA), a residual contaminant derived from plastic materials, on binary mixtures of the phospholipid 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and cholesterol (Chol). These mixtures were structured into bilayers, forming giant unilamellar vesicles (GUVs), and monolayers, forming Langmuir films, whose compositions and phases are relevant for mimicking the cell plasma membrane in eukaryotic cells. The composition of the binary mixture of DOPC/Chol 8:2, representing the proportion of the primary lipid groups found in cells, was effective in inducing inward buddings when exposed to BPA, in addition to the multiple shape transformations observed for DOPC vesicles. In monolayers, BPA induced the pi-A isotherm expansion of DOPC, Chol, and the binary mixtures, also mainly in the DOPC/Chol 8:2 monolayers. Consistently, polarization-modulated infrared reflection absorption spectroscopy measurements revealed more pronounced interactions between BPA and the DOPC headgroup (hydrogen bonding from the hydrogen of the BPA phenol group and the nitrogen from the DOPC choline group, preferentially) in the DOPC/Chol 8:2 monolayer compared to that in individual DOPC monolayer. The presence of Chol appears to make the DOPC headgroups more accessible for interaction, even in bilayers. These results emphasize the substantial impact of BPA on the DOPC/Chol binary mixture, highlighting its critical role in predicting the potential damage to human cell membranes. (AU) | |
| FAPESP's process: | 18/22214-6 - Towards a convergence of technologies: from sensing and biosensing to information visualization and machine learning for data analysis in clinical diagnosis |
| Grantee: | Osvaldo Novais de Oliveira Junior |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 21/14235-6 - Membrane mimetic models applied in interaction studies, detection, and removal of emerging pollutants |
| Grantee: | Priscila Alessio Constantino |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 20/15185-0 - Lipid nanoparticles for encapsulation and carrier of natural products: methods of preparation, comparison and optimization |
| Grantee: | Gilia Cristine Marques Ruiz |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 23/00291-7 - Effects of the food colorant Allura Red AC on mimetic systems of the lipid structure of the cell membrane of the gastrointestinal tract |
| Grantee: | Carlos Junior Amorim Lessa |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |