Impact of Sleeve Gastrectomy on Body Weight and Food Intake Regulation in Diet-Induced Obese Mice

Author(s)

Balbo, Sandra Lucinei ; Soares, Gabriela Moreira ; Morari, Joseane ; Felisberto Jr, Antonio Machado ; Vettorazzi, Jean Franciesco ; Bronczek, Gabriela Alves ; Bonfleur, Maria Lucia ; Carneiro, Everardo Magalhaes ; Boschero, Antonio Carlos ; Velloso, Licio Augusto

• Document type: Journal article
• Source: CURRENT ISSUES IN MOLECULAR BIOLOGY; v. 46, n. 11, p. 8-pg., 2024-11-01.

Abstract

The epidemic of obesity has increased worldwide and is associated with comorbidities such as diabetes and cardiovascular disease. In this context, strategies that modulate body weight and improve glycemic metabolism have increased, and bariatric surgeries such as Sleeve Gastrectomy (SG) have been highlighted in obesity treatment. However, the mechanism by which SG reduces body weight and improves glycemic control remains unknown. Thus, in this study, we aimed to evaluate food intake and the expression of hypothalamic genes involved with the regulation of this process in diet-induced obese mice submitted to SG. For this, we used C57BL/6 mice submitted to a 10-week high-fat diet protocol and submitted to SG. Food intake, fed and fasted glycemia, as well as hypothalamic anorexigenic and orexigenic gene expression were evaluated 4 weeks after the surgical procedure. First, we observed that SG reduces body weight (44.19 +/- 0.47 HFD, 43.51 +/- 0.71 HFD-SHAM, and 38.22 +/- 1.31 HFD-SG), fasting glycemia (115.0 +/- 4.60 HFD, 122.4 +/- 3.48 HFD-SHAM, and 93.43 +/- 4.67 HFD-SG), insulinemia (1.77 +/- 0.15 HFD, 1.92 +/- 0.27 HFD-SHAM, and 0.93 +/- 0.05 HFD-SG), and leptinemia (5.86 +/- 1.38 HFD, 6.44 +/- 1.51 HFD-SHAM, and 1.43 +/- 0.35 HFD-SG) in obese mice. Additionally, SG reduces food (5.15 +/- 0.18 HFD, 5.49 +/- 0.32, HFD-SHAM, and 3.28 +/- 0.26 HFD-SG) and total (16.88 +/- 0.88 HFD, 17.05 +/- 0.42, HFD-SHAM, and 14.30 +/- 0.73 HFD-SG) calorie intake without alterations in anorexigenic and orexigenic gene expression. In conclusion, these data indicate that SG improves obesity-associated alterations at least in part by a reduction in food intake. This effect is not associated with the canonical food intake pathway in the hypothalamus, indicating the involvement of non-canonical pathways in this process. (AU)

• FAPESP's process: 13/07607-8 - OCRC - Obesity and Comorbidities Research Center
• Grantee: Licio Augusto Velloso
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 15/12611-0 - Molecular mechanisms involved in pancreatic beta cell disfunction and dead in diabetes mellitus: strategies for the inhibition of these processes and restoration of the insular mass
• Grantee: Antonio Carlos Boschiero
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 21/04664-7 - Molecular mechanisms involved in pancreatic beta cell dysfunction and death in Diabetes Mellitus: strategies for the inhibition of these processes and restoration of the insular mass
• Grantee: Antonio Carlos Boschiero
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 19/00728-0 - The role of gut-pancreas axis upon glycemic homeostasis maintenance in obese mice submitted to vertical gastrectomy: a molecular and functional approach
• Grantee: Gabriela Moreira Soares
• Support Opportunities: Scholarships in Brazil - Post-Doctoral